PROJECT STEALTH                         
May 29, 2014


Thank you again for your interest and commitment to Project Stealth and this

innovative Cancer Research Program. I last updated you several months ago

and I am very excited to share our progress with you.


As we understand how cancers develop and spread, we and numerous

other researchers are focused on the "tumor microenvironment". This is the

environment within a cancer that allows cancers to grow and flourish. Project

Stealth is a research program that capitalizes on a finding that Salmonella

has a unique ability to stealthily invade cancers. We are using these bacteria,

which have been genetically altered so they cannot cause any side effects

and to deliver a cocktail of immune modulating proteins right to a cancerous

tumor, essentially into the tumor microenvironment. The proteins we are

delivering stimulate the immune system to make cancer killing immune cells. In

addition, we have engineered salmonella to carry proteins that block a cancer's

ability to suppress the immune system. Thus, we are altering the cancer's

microenvironment by simultaneously stimulating the immune system to kill

cancer and stop the cancer cell's ability to spread and grow.


As of yesterday, Project Stealth has raised $202,038.00 and is getting closer to

our overall goal of $500,000.00. Those funds are currently being used to study a

variety of cancer types. At this point, we are primarily focused on Pancreas and

Breast Cancer and want to expand to many more as the research dollars allow

us to.


There are many models of breast cancer in mice. We are choosing to study our

treatment strategy with one of the breast cancer models that is closest to what

is seen in humans. This model is both expensive (about $100 per mouse) and

difficult to maintain; however, we strongly believe in this approach because we

know that these results will have the ability to translate to people. These mice

carry a breast cancer gene and 100% of them will develop breast cancer. We

are waiting to treat these mice until we can feel the cancer. As you can see, by

waiting to treat a large tumor, we are "stacking the deck" against us to really test

what we have. Here are preliminary results; Control mice are represented by the

black line and are dead from breast cancer within 30 to 40 days after one can feel a

breast cancer. Looking at various combinations of Salmonella, if we give these

mice Salmonella that contain the immunostimulatory protein, IL-2, and tumor

microenvironment blocking proteins, aPD-1 and aCTLA-4, we see these mice

surving much longer and keeping the breast cancers very small.





We still have much to do; we need to determine how much Salmonella to give,

how often to give it, and if there is a different combination that would be even

more effective. In addition, the FDA will require a host of studies once we decide

which combination to go with in our Investigational New Drug (IND) Application.

As you can see, these results are very exciting. Your enthusiam and support for

Project Stealth drives us each and every day to carry this to find a solution for

this dreaded disease.


Thank you for your support. Again, please share our website www.projectstealth.org
with your family and friends.

Sincerely,

Daniel Saltzman, M.D., PhD.

Associate Professor of Surgery and Pediatrics

Chief, Division of Pediatric Surgery

Surgeon-in-Chief, University of Minnesota Children's Hospital

(612) 626-4214