Pre-weaning animals that die or are euthanized without any experimental manipulation (except for genotyping) should notbe counted against the total number of animals allocated by the IACUC approved protocol. In other words, if a pre-weaning animal is genotyped and euthanized because it does not have the appropriate phenotype, it should notbe deducted from the number of animals approved by the IACUC for the study.
However, pre-weaning animals that undergo experimental procedures other than genotyping must be counted against the total number of animals approved by the IACUC for the study.
All weaned animals, regardless of use, must be counted against the total number of animals specified in the IACUC-approved protocol.