Researchers ID ecological frog 
deterrents  for  leafy green fields

Key Take-Aways
* A corrugated metal barrier with a 10-centimeter lip can significantly reduce frog movement into fields.
* The barriers also work on small mammals, lizards and other small trespassers.
Contact local wildlife experts to learn about frog biology as it may differ from some books.
June 9, 2020  - Food safety research is often about the contamination that an animal leaves after they have visited a produce field. This project focuses on animals that live in the environment and are small enough to be caught up in the harvesting of an area and end up in a fresh-cut product.   

To help growers and processors minimize these incidents, researchers have found a lipped barrier made of aluminum siding can prevent Pacific tree frogs from moving into leafy green fields. Even silt fences, common in California's Salinas Valley, can reduce frog movement by nearly half, said Michelle Green, Ph.D., University of South Florida in St. Petersburg and project lead.

"It's important for growers to know that while any fence is better than no fence, we can offer ways to improve the fences they use," she said.

The two-year project, titled "Engineering and ecological approaches reduce Pacific tree frog intrusion into leafy green agriculture," evaluated several methods for reducing frog interlopers into fields, which included barriers and pre-recorded frog calls. 

Joining Green as co-principal investigators were Paul Davidson, Ph.D., Assistant Professor of Agricultural and Biological Engineering with the University of Illinois Urbana-Champaign (UIUC), and Jonathan Warner, Ph.D., Alligator Program leader with the Texas Parks and Wildlife Department.

Michelle Green,
PhD, University of South Florida in  St. Petersburg
The industry-preferred method for excluding frogs is erecting silt or drift fences - low-stature woven-fabric fences typically used for erosion control - around leafy green fields.

Based on input from industry, the researchers learned that potential barriers had to be economical and withstand strong winds that typically come up in the afternoon in the Salinas Valley.

Daniel Hughes, Ph.D. and a postdoctoral researcher with the UIUC Department of Animal Sciences, initially conducted field trials akin to escape rooms. He put frogs in enclosures constructed of various materials to measure how many were able to climb over the top.

Based on the trial results, the researchers narrowed candidate materials down to aluminum siding with a 10-centimeter lip at the top, a sandpaper-covered fence and window screening. The silt fencing acted as the control.

Working with Salinas Valley cooperators, Hughes then erected the short-stature barriers around natural frog habitat near leafy green fields and monitored how many frogs climbed out.

About 60% of the frogs were able to scale the window screening, and 55% made it over the silt fencing. But only 8% were able to climb over the sandpaper barrier, and none made it over the aluminum siding.

Davidson said the two top barriers work in different ways. Pacific tree frogs use suction-cup-like toe pads and secrete a liquid to aid suction as they climb. The sandpaper's rough texture reduces the efficacy of the frog's suction system.

The aluminum barrier - and particularly the lip - creates an uncomfortable environment for frogs, which don't like to be in the open, Green said. The frogs that did climb the aluminum barrier hid under the lip and never made it over the top.

The aluminum barrier also was effective on small mammals, lizards and other small trespassers, she said.

Part of the project looked at whether pre-recorded frog calls could be used to prompt frog movement. Although the results were promising, Green cautioned they were only from three sites.

"There was one male that would position itself right by the speakers," she said. "They do respond to the vocals. There would be no calls but when the recorded calls started, it induced activity and that could potentially get the frogs to move. Then the question becomes, where do you want them to move to?"

The researchers also sought to learn more about the Pacific frog biology to determine when they were the most active and potentially could move into fields.

"The take-away  is we saw activity at different times of the year than expected," Green said. In February and March, for example, full-sized adults were ramping up to breed. This differs from a guide book that said Pacific tree frogs are most active in November and December.

"It helps to know when frogs are likely to be active and near their fields so growers can decide what type of fencing to use," Green said. "They can put in fencing that will be permanent or they can do something that is temporary."

The methods also may reduce frogs in the growing environment without negative impacts to wildlife in the area.

The researchers credit their Salinas Valley cooperators and the California Leafy Greens Research Program  advisors for their project's success.

"I don't know if we could have done the study without the cooperators," Davidson said. "They helped us find sites for the study. They gave Danny and our whole team access and provided people to help install the fences. They did more than we could have asked."
______________________________________________________________________________________ ____

Project abstract:

Michelle Green, PhD, University of South Florida, St. Petersburg
Michelle Green, PhD, 
University of South Florida in St. Petersburg

CPS minute video with Dr. Green may be found on the  CPS YouTube channel  as well as the Videos page on the CPS website

Learn more about this project and other CPS funded research by attending the 2020 CPS Research Symposium Webinar Series. For more information and updates, please visit the CPS Symposium webpage.
Fund the science, find solutions and fuel the change.
About CPS
The Center for Produce Safety (CPS) is a 501(c)(3), U.S. tax-exempt, charitable organization focused exclusively on providing the produce industry and government with open access to the actionable information needed to continually enhance the safety of produce.   
For more information:
Email: . Phone:  530-554-9706