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Data Analysis Skills for Chromatographers
November 17; 8:30am – 5:00pm
Instructors: Merlin Bicking, ACCTA, Inc.
Course Description:
We all need to analyze our acquired HPLC data, but there is surprisingly little information and training available. This course will help new and intermediate-level analysts get started and become more efficient with their Data Analysis activities. We focus on the three critical aspects of chromatographic Data Analysis – examining your chromatography, integrating the peaks of interest, and using calibration standards to calculate the quantities of our analytes. Serious analytical errors can result from a failure to follow best practices in any of these three activities. Real-world examples are used throughout this course to help teach these concepts.
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Chromatographic Methods of Analysis of Oligonucleotides, siRNA, sgRNA and mRNA
November 16: 8:30am - 5:00pm
Instructor: Martin Gilar, Waters Corp.
Course Description:
This course will cover the development of liquid chromatography (LC) methods for analysis of nucleic acid therapeutic compounds. Ion-pair reversed-phase LC (IP RP LC) or hydrophilic interaction chromatography (HILIC) are method compatible with mass spectrometry (MS) detection. Size exclusion chromatography (SEC), ion-exchange chromatography (IEC) and affinity LC are useful for routine quality control with UV detection. We will describe the method development for analysis of antisense oligonucleotides (ASO), silencing RNA (siRNA), short guide RNA (sgRNA) and messenger RNA (mRNA). In addition, we will illustrate the impact of non-specific interactions with LC metal hardware on oligonucleotide quantitation, tailing, and carryover. Click here for more details.
Getting the most from GC and GC/MS
November 17; 8:30am – 5:00pm
Instructors: Gregory Slack, Consultant & Nicholas Snow, Seton Hall University
Course Description:
Gas chromatography (GC) and GC/mass spectrometry (MS) are staple techniques in chemical analysis. This course will enable users of GC and GC/MS to extend the power of their instruments to separate wider ranges of compounds with better sensitivity and fewer problems. Specific sessions will focus on method optimization for speed, sensitivity and/or ease-of-use, instrument set-up and qualification, troubleshooting and maintenance and effective combination of sampling, sample preparation, separation and detection to maximize method performance. Attendees are encouraged to bring example problems from their own work for discussion.
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Systematic HPLC Troubleshooting
November 18; 8:30am – 5:00pm
Instructors: Merlin Bicking, ACCTA, Inc.
Course Description:
If you just need to get better at troubleshooting your HPLC system, then this one-day course is for you. No complex theory discussions, just lots of practical information for solving problems. Taught by a practicing chromatographer, this course highlights routine system operation and its role in preventing future problems. The discussion makes extensive use of case studies and examples to help students learn by examining real-world problems and solutions. We finish with the famous “LC Quiz,” a group problem-solving discussion where we explore selected problems and work through the steps necessary to solve each issue. It’s the students’ favorite.
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HPLC and UHPLC for Practicing Scientists 1 and 2: Fundamentals, Method Development, and Troubleshooting
November 16 and 17; 8:30am – 5:00pm
Instructor: Michael Dong, MWD Consulting
Course Description:
This intermediate course will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns, applications, and best practices in method development and troubleshooting of HPLC and UHPLC (ultra-high-pressure liquid chromatography). The focus is on pharmaceutical analysis of small molecule drugs. This course can also be taken as Day/Part 1 only or Day/Part 2 only. Click here for more details.
Practical LC-MS/MS Method Development & Sample Preparation
November 16 and 17; 8:30am – 5:00pm
Instructor: Perry Wang, LC-MS Technical Expert
Course Description:
This course offers practical training for practical scientists. It will take the participants step‐by‐step through the concepts and techniques to develop LC‐MS methods. The emphasis is on practical issues associated with developing LC‐MS methods for small molecules. It also emphasizes problem‐solving skills with examples encountered in the pharmaceutical industry and other fields. This course will provide the participants with an updated overview and a solid working knowledge of LC‐MS. The participants will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guides, and key applications. After this course, the participants will be able to independently develop their own LC‐MS methods. New technologies and techniques, such as monolithic chromatography and hydrophilic interaction liquid chromatography (HILIC) will be presented.
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November 18; 8:30am – 5:00pm
Instructor: Perry Wang, LC-MS Technical Expert
Course Description:
This short course is designed to offer practical training for the practicing scientists in the analytical field. Validation of analytical methods is crucial for the successful conduct of nonclinical, biopharmaceutics, and clinical pharmacology studies. This short course will focus on the FDA’s Bioanalytical Method Validation – Guidance for Industry published in May 2018 using LC-MS/MS. It will take the participants step-by-step through the concepts and techniques to validate bioanalytical methods. The emphasis is on practical issues associated with the bioanalytical method validation. The participants will learn the essence of the method validation. After this course, the participants will be able to independently validate their own bioanalytical methods. How to apply the validated methods for routine assay and some typical case studies will be presented as well. Click here for more details.
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