Mitegen October Newsletter
Paper - High-resolution Single-particle Cryo-EM of Samples Vitrified in Boiling Nitrogen
Image Source: Thorne, et al. (2021). High-resolution single-particle cryo-EM of samples vitrified in boiling nitrogen. IUCrJ, volume 8, Part 6
A paper at IUCrJ by Robert E. Thorne, et al., demonstrates how "single-particle cryo-EM samples on commercial grids can be routinely vitrified using only boiling nitro­gen and obtain apoferritin datasets and refined structures with 2.65 Å resolution."

This avoids the use of ethane, propane or ethane/propane mixtures as liquid cryogens. By using liquid nitrogen as the primary coolant, this "may allow manual and automated workflows to be simplified and may reduce sample stresses that contribute to beam-induced motion."
NANUQTM Advanced Cryocooling Device
Our NANUQTM Advanced Cryocooling device was used to plunge cool samples as part of the research mentioned in the above paper.

NANUQTM is designed to replace hand plunging of crystals, and was repurposed in the above paper for use in cryo-EM sample preparation.
Paper - Michaelis-like Complex of SARS-CoV-2 Main Protease Visualized by Room Temperature X-ray Crystallography
From a paper at IUCrJ by Daniel W. Kneller, et al., read about how the SARS-CoV-2 main protease and substrate complex were captured by room-temperature X-ray crystallography.
New Product - Thermal Shipper For Room Temperature Crystallography Samples
The thermal shipper is a reusable temperature controlled shipping container used to safely transport room temperature crystallography samples in a controlled environment. Designed to hold up to six SSRL-in-situ crystallization plates securely at 20 (=5 / -5) degrees Celsius for up to 168 hours.
Small Molecule Crystallography Survey
Small molecule crystallographers, help us understand your needs better. Please take our short survey which asks just a few questions. Share your knowledge to help us serve you better in your research.

Your answers and information will remain confidential.
Upcoming Events
At MiTeGen, we sponsor numerous events, conferences, workshops and symposiums throughout the year. These events include fields such as structural biology, crystallography, cryo-EM, beamline research and more. We believe in supporting researchers and our industry. Listed below are two upcoming events you might find interesting.
The Annual Buffalo-Hamilton-Toronto (BHT) Crystallography-CryoEM Symposium is November 5th. Format is virtual. Features new and innovative techniques in X-ray crystallography and cryoEM, and provides a venue for showcasing the achievements of graduate students and postdoctoral fellows. We are sponsoring this event.
The 6th Annual Southern California Cryo-Electron Microscopy Symposium is October 29th. Format is virtual. Brings together electron microscopists for talks and poster sessions on studying the fundamental mechanisms of biological systems. We are sponsoring and giving a talk at this event.
Looking For More Events, Workshops and Conferences
If you are looking for more events visit our events page. It lists conferences, workshops, symposiums, user meetings and more.

Topics include structural biology, material science, crystallography, cryo-EM, beamline research and more.
Industry Resources and News
Here is a small selection of hand picked industry resources and news for your browsing.

  • Read about updated image integrity guidelines at EMBO Press. Includes checks for image aberrations, image integrity principles, journal image integrity checks and classifications of image aberrations - Learn More
  • Diamond Light Source offers a podcast series called Shining A Diamond Light. Through it you can learn about synchrotron science and the ground-breaking research that happens every day at their world-leading facility - Learn More
  • The HZB Highlight Report offers the most important scientific highlights of the HZB research center and gives an interesting overview of the research successes achieved in 2019 and 2020 - Learn More
  • If you are interested in microscopy, the Microscopist biweekly podcast features talks with leading microscopist. It is sponsored by Zeiss and episodes are released every two weeks - Learn More
  • Registration for the Hercules School at the European Synchrotron is now open. Spend a month learning about X-rays, neutrons and all the possible experimental techniques - Learn More 
  • The following thread on Twitter walks you through the steps concerning, “new research on the discovery of peptide inhibitors of SARS-CoV-2 Mpro which was made possible in part by early data from Diamond and with collaboration with others.” - Learn More
  • Applications are now being accepted for ERC Starting Grants. These are designed to support Principal Investigators at the career stage at which they are starting their own independent research team or program - Learn More
  • Instruct Eric is accepting applications for access to any of their facilities - Learn More
  • The Fall ALS Colloquium has started. Hear the latest updates on accelerator technology, microchips, CRISPR, quantum materials, and SARS-CoV-2 antibody therapeutics - Learn More
  • Read about the 8th edition of Beamline for Schools which ended on September 21st. Two teams spent two weeks at DESY working on their experiments and to experience what it’s like to be a scientist - Learn More
Industry Jobs
Searching for a new job? Interested in knowing what positions labs and facilities are offering? Then visit our jobs board on which we list recent industry job offerings.

And remember, if you have an industry relevant job, we are happy to list it on our jobs board.
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