WHAT'S NEW WITH DART ? MS?                                       May 2015   
 The Latest Developments in Direct Analysis in Real Time Mass Spectrometry 

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We have just been made aware that DART-MS has been proposed in a method for screening of dietary supplements (DS) for the presence of Erectile Dysfunction drugs. 
As part of the USP Global Education and Trainig program they have invited you to join their Live Webinar Thursday (tomorrow) from 12:00 - 1:00pm.  Anton Bzhelyansky of USP and Dr. Teresa Cain of the FDA will discuss why "screening" is seen as an analytical modality of choice when adulteration of botanicals is suspected.

Click here to REGISTER for the Webinar.  Use this code 225USP25 to receive a discount of 15% off the $95 fee.
If you plan to attend the ASMS this coming month in St. Louis you can also see our poster focused on screening of supplements for PDE-5 Inhibitors.  We have recently submitted our work to the AOAC as a potential method for screening and confirmation of those dangerous drugs which are now appearing as adulterants in everyday supplements.

Feel free to contact me if you have any questions or comments. 

Best regards,

Brian Musselman, Ph.D.
President and CEO

Recent DART Publications on Adulterants

The Complete DART Bibliography is Now Available - CLICK HERE
Upcoming ASMS Abstract - Tuesday Poster 352


Rapid Screening and Confirmation of PDE5 Inhibitors in Dietary Ingredients by DART-MS Ambient Ionization


Robert Goguen, Julie Carbonello, Elizabeth Crawford, and Brian Musselman


IonSense, Inc., Saugus, MA 01906, USA


Phosphodiesterase type 5 inhibitors (PDE5 Inhibitors) are used to treat erectile dysfunction (ED). Numerous different PDE5's have been detected in herbal supplements, vitamin tablets and adulterated pharmaceuticals. In response to this adulteration problem the AOAC has issued a call for methods focused on development of technologies for rapid determine the presence of various PDE5s in dietary ingredients. This work describes the method we have developed to address this adulteration problem.



Analysis of PDE-5 inhibitor standards at multiple concentrations was completed using a Direct Analysis in Real Time (DART-SVP) ambient ionization source coupled to both a HRMS and a QQQ LC/MS system. Samples and standards were prepared for analysis by dissolving a quantity with solvents. For preparation of adulterated materials, an aliquot of each standard solution was applied directly to the supplement material and allowed to dry before extraction. 33 replicates of each supplement were analyzed. The [M+H]+ with accurate mass assignment was used to confirm PDE5s presence in the screening method. Statistical analysis of the accuracy of the method was assessed for each of the analytes in each type of sample. An MS/MS method was used for the confirmation method.


 DART-MS for rapid, preliminary screening of urine for DMAA 


Ashton D. Lesiak, Kendra J. Adams, Marek A. Domin, Colin Henck and Jason R. E. Shepard

Boston College and University of Albany


Dimethylamylamine (DMAA) is a sympathomimetic amine found in weight-loss/workout supplements or used as an appetite suppressant. DMAA is a stimulant that is banned by the World Anti-Doping Agency (WADA). Adverse health effects as well as fatalities have been implicated with its use. Direct analysis in real time mass spectrometry (DART-MS) is an ambient ionization method that was employed to rapidly identify the presence of DMAA in various samples without any extraction or preparations whatsoever. DMAA was first identified in supplements, sampled directly in their solid forms. Furthermore, DMAA was detected directly in urine over 48 h as a means of indicating recent abuse of the substance. DART-MS analysis is instantaneous, and coupled with the high mass accuracy associated with the time-of-flight mass analyzer, results in unequivocal identification of the presence of DMAA. These features demonstrate DART-MS as an attractive potential alternative screening method for the presence of drugs and medications or for toxicological investigations. Copyright ? 2013 John Wiley & Sons, Ltd.




Just dip it: online coupling of "Dip-it" polymer monolith microextraction with plasma assisted laser desorption ionization mass spectrometry



Xin Wang, Xianjiang LiYu Bai and Huwei Liu 

Beijing University   

A polymer monolith microextraction (PMME) procedure coupled to plasma assisted laser desorption ionization mass spectrometry (PMME-PALDI-MS) was developed for rapid and organic solvent-free trace analysis. The extraction device used a "Dip-it" sampler coated with a MWNT incorporated monolith, and the analytes adsorbed on monoliths were effectively desorbed by laser, improving detection sensitivity. 

Rapid screening for synthetic antidiabetic drug adulteration in herbal dietary supplements using direct analysis in real time mass spectrometry

Zhigui ZhouJialing Zhang, Wei Zhang, Yu Bai and Huwei Liua  



Adulteration of herbal supplements with synthetic drugs is illegal. A rapid and reliable method which utilizes direct analysis in real time mass spectrometry (DART-MS) was developed for the identification of seven synthetic antidiabetic drugs used as adulterants in herbal dietary supplements. The supplement sample was simply extracted with methanol/water by manually shaking several times and directly analyzed using DART-MS. The presence of synthetic drug adulterants was confirmed through the accurate m/z values and MS/MS data obtained via quadruple time of flight mass spectrometry (QTOF MS). Parameters for the DART source were systematically optimized, and the limits of detection (LODs) in herbal supplement matrices were measured. This method was successfully applied to examine five commercial herbal dietary supplements, and two of them proved to be adulterated with metformin without labeling.







About IonSense
IonSense, Inc. provides OpenSpot Mass Spectrometry? solutions to the fields of food safety, forensics, drug development, and chemical analysis. We manufacture and develop direct analysis in real time (DART ?) technology licensed from JEOL USA, Inc. and atmospheric solids analysis probe (ASAP?) licensed from M&M Consulting.

DART and ASAP Sources are available for most commercial LC/MS systems.  Look here to see if your system is DART-ready.  And   check here to see if your system is ASAP-ready.

For your local representative, please see our distributor network.

Phone - 781.484.1043  | |