The Power of GFP in Neuroresearch

Illuminate GFP with Biosensis Antibodies

Biosensis offers GFP antibodies from different hosts: mice, rabbits, chickens, and goats. These antibodies are superb for GFP detection and react with various GFP variants, including eGFP and AcGFP. They effectively verify GFP protein expression, size, and stability in all typical applications and detect GFP excellently in most fixation conditions, including paraffin tissues. Thus, they provide researchers with a cornucopia of choices and versatility unmatched by other firms.

GFP,Rabbit Polyclonal Antibody (R-1838-100)

Above, left: Analysis of GFP expression in transfected HEK293 cells by fluorescent microscopy (green). The same cells were stained with rabbit anti-GFP antibody (red, R-1838-100) labeled with a red fluorochrome by fluorescent immunocytochemistry. The blue color is Hoechst staining for nuclei. Transfected cells appear orange-yellow because of the green (GFP) natural fluorescence and red (anti-GFP antibody) color overlay. 

Above right: Western blot analysis of GFP protein in transfected HEK293 lysate using a rabbit antibody to GFP (green, R-1838-100). The band at ~27kDa corresponds to GFP protein, the upper red bands are a tubulin protein detected as a loading control using an anti-tubulin antibody.

GFP, Goat Polyclonal Antibody (G-2103-100)

Above, left: Analysis of GFP fusion protein-transfected HEK293 cells by fluorescent microscopy (green). GFP expression was simultaneously detected with goat antibody to GFP labeled with a red fluorochrome (red, G-2103-100) by immunocytochemistry. Blue: Hoechst nuclear stain. The goat antibody reveals GFP protein expressed only in transfected cells, which results in these cells appearing yellow in color caused by overlapping colors.

Above, right: Western blot analysis of GFP expression (green, G-2103-100) in transfected HEK293 cells with goat anti-GFP antibody. GFP migrates at ~ 27kDa.

GFP,Chicken Polyclonal Antibody (C-2102-100)

Above Left: Analysis of GFP fusion protein-transfected HEK293 cells by fluorescent microscopy and immunocytochemistry. The fluorescing GFP protein (green) was detected with a chicken antibody to GFP labeled with a red fluorochrome (red, C-2102-100). Blue: DAPI nuclear stain. The chicken antibody reveals GFP protein expressed only in transfected cells, and the overlap of the two fluorochromes appears yellow when visualized together. Above Right: Western blot analysis of GFP expression (green, C-2102-100) in transfected HEK293 cells with chicken anti-GFP antibody. The band at ~27kDa corresponds to GFP protein, the upper red bands are a tubulin protein detected as a loading control using an anti-tubulin antibody.

GFP,Mouse monoclonal Antibody (M-2104-100)

Above: The Biosensis monoclonal antibody M-2104-100 against GFP was used together with DAB colorimetric mouse brain (brown) to detect GFP protein. The GFP antibody exhibited selective labeling of cells in the cerebellum, as anticipated, and the counterstain employed was hematoxylin, which imparts a blue color to the surrounding tissues.

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