IMBeR IPO-China 信息速递
Your news from the Integrated Marine Biosphere Research International Project Office - China
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IMBeR IPO - China is fully sponsored by ECNU and SKLEC
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Editors: GiHoon HONG, Kai QIN and Fang ZUO from IMBeR IPO
Academic adviser: Shan JIANG from SKLEC, ECNU
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黄海沿岸悬挂式水产养殖对水动力和营养盐供给的影响
Influence of Suspended Aquaculture on Hydrodynamics and Nutrient Supply
in the Coastal Yellow Sea
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作者:Yuqing He, Jiliang Xuan, Ruibin Ding, Hui Shen, and Feng Zhou
期刊:Journal of Geophysical Research: Biogeosciences
沿海养殖业已成为许多国家人类消费海产品的主要来源,中国对全球水产养殖产量的贡献率达到60%。黄海沿岸的养殖场作为中国水产养殖产量的最大贡献者,近年来已经扩展到20-30米深度的水体。过度的水产养殖导致了与水动力减弱相关的营养盐缺乏。本文通过对黄海沿岸典型水产养殖场的营养盐观测和高分辨率模拟,研究了悬挂式水产养殖对水动力和相关营养盐供给的影响。研究结果表明,营养盐供给显著减少与越过养殖边界的向岸流变化、近海养殖区(>10 m)的上升流变化和近海岸养殖区(<10 m)的水交换变化有关。具体而言,悬挂式水产养殖削弱了养殖边界附近的向岸流和近海养殖区内的上升流,改变了海湾边界附近向岸流的垂向分布。因此,近海养殖区高营养盐水体减少约60%。
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Figure 1: (a) Geographic location of a typical coastal aquaculture area in the Yellow Sea (red rectangle). The red dots indicate the locations of the suspended aquaculture sites along the coast. The pink contours indicate tidal fronts in the Yellow Sea. The blue shaded area indicates the Yellow Sea cold-water mass in the spring. (b) Sanggou Bay and its adjacent area viewed on Google maps. The black curve shows the aquaculture boundary. White boxes indicate the near-coastal and offshore aquaculture areas, respectively. (c) Diagram of natural frontal circulation and the effects of suspended aquaculture on currents and boundary circulation.
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Coastal aquaculture has become the largest source of seafood for human consumption in many countries, and China contributes 60% of global aquaculture production. As the largest contributor to Chinese aquaculture production, the coastal aquaculture sites in the Yellow Sea have expanded to depths between 20 and 30 m in recent years, while excessive aquaculture results in nutrient deficiency that has been associated with weakened hydrodynamics. Through nutrient observations and high-resolution simulations at a typical aquaculture site, we investigated the impact of suspended aquaculture on hydrodynamics and the associated nutrient supply in the coastal aquaculture area of the Yellow Sea (China). Our results show that a significant reduction in nutrient supply, which is associated with alterations in onshore currents across the aquaculture boundary, upwelling within the offshore (>10 m) aquaculture area, and water exchange in the near-coastal (<10 m) aquaculture area. Specifically, suspended aquaculture weakens the onshore current near the aquaculture boundary and upwelling within the offshore aquaculture area and changes the vertical distribution of the onshore currents near the bay boundary. As a result, approximately 60% of the high-nutrient water in the offshore aquaculture area is reduced.
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贻贝粘合剂启发的类蛋白聚合物
Mussel Adhesive-inspired Proteomimetic Polymer
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作者:Or Berger, Claudia Battistella, Yusu Chen, Julia Oktawiec, Zofia E. Siwicka, Danielle Tullman-Ercek, Muzhou Wang, and Nathan C. Gianneschi
期刊:Journal of the American Chemical Society
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本文介绍了一种合成的类蛋白聚合物,该聚合物重构了贻贝粘附蛋白的关键结构元素和功能,以降冰片烯基肽单体为原料,通过嫁接-穿梭(graft-through)开环易位聚合法制备而成。这种肽源于海洋贻贝分泌的天然水下粘合剂,由高度重复的十肽序列串联组成。作者假设,以这种方式再现重复单元将为制备受自然启发的粘合剂提供一条简便的途径。为了达到这个目的,研究人员对这种材料进行了检测,并将其与天然蛋白质进行了比较:这种材料的肽单元的排列类似刷状聚合物的侧链,而非像天然蛋白质那样以线性方式排列。对固体基底的粘附能力的机械测量显示,这种材料具有比天然蛋白质更好的粘附能力,这使得该项工作具有广泛的应用前景。作者还展示了使用此聚合物作为固定活细胞的表面粘合剂的应用。
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Herein, a synthetic polymer proteomimetic is described that reconstitutes the key structural elements and function of mussel adhesive protein. The proteomimetic was prepared via graft-through ring-opening metathesis polymerization of a norbornenyl-peptide monomer. The peptide was derived from the natural underwater glue produced by marine mussels that is composed of a highly repetitive 10 amino acid tandem repeat sequence. The hypothesis was that recapitulation of the repeating unit in this manner would provide a facile route to a nature-inspired adhesive. To this end, the material, in which the arrangement of peptide units was as side chains on a brush polymer rather than in a linear fashion as in the natural protein, was examined and compared to the native protein. Mechanical measurements of adhesion forces between solid surfaces revealed improved adhesion properties over the natural protein, making this strategy attractive for diverse applications. One such application is demonstrated, using the polymers as a surface adhesive for the immobilization of live cell.
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新型胰岛素诱导的对称和不对称受体构象连续体
Symmetric and Asymmetric Receptor Conformation Continuum
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作者:Xiaochun Xiong, Alan Blakely, Jin Hwan Kim, John G. Menting, Ingmar B. Schäfer, Heidi L. Schubert, Rahul Agrawal, Theresia Gutmann, Carlie Delaine, Yi Wolf Zhang, Gizem Olay Artik, Allanah Merriman, Debbie Eckert, Michael C. Lawrence, Ünal Coskun, Simon J. Fisher, Briony E. Forbes, Helena Safavi-Hemami, Christopher P. Hill, and Danny Hung-Chieh Chou
期刊:Nature Chemical Biology
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导言:跨学科研究的案例——从海洋生物锥形蜗牛麻痹鱼的毒液,到用于糖尿病治疗的新的速效胰岛素
锥形蜗牛毒液含有多种生物活性肽,包括具有独特结构特征、结合模式和生化特性的类胰岛素分子。本文报告了一种活性的人源化(与人类胰岛素相似的)锥形蜗牛毒液胰岛素,它具有一个延伸的A链和一个截短的B链,并使用冷冻电子显微技术(cryo-EM)和蛋白质工程来阐明其与人类胰岛素受体(IR) 胞外域的相互作用。作者揭示了一个延伸的A链如何弥补B链残基的缺失,这对人类胰岛素的活性至关重要,但也会因皮下注射部位的延迟溶解而损害治疗效用。这一发现提出了开发改进的治疗性胰岛素的方法。不寻常的是,受体表现出从对称状态到高度不对称的低丰度结构的连续构象,这一结构利用先前表征的位点1和位点2相互作用的元素,显示出单个人源化毒液胰岛素的协调作用。
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Introduction: A typical case of interdisciplinary research – from cone snail venoms for paralyzing fish to new fast-acting insulin in diabetes treatments
Cone snail venoms contain a wide variety of bioactive peptides, including insulin-like molecules with distinct structural features, binding modes and biochemical properties. Here, we report an active humanized cone snail venom insulin with an elongated A chain and a truncated B chain, and use cryo-electron microscopy (cryo-EM) and protein engineering to elucidate its interactions with the human insulin receptor (IR) ectodomain. We reveal how an extended A chain can compensate for deletion of B-chain residues, which are essential for activity of human insulin but also compromise therapeutic utility by delaying dissolution from the site of subcutaneous injection. This finding suggests approaches to developing improved therapeutic insulins. Curiously, the receptor displays a continuum of conformations from the symmetric state to a highly asymmetric low-abundance structure that displays coordination of a single humanized venom insulin using elements from both of the previously characterized site 1 and site 2 interactions.
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粒子觅食策略促进海洋环境中微生物多样性
Particle Foraging Strategies Promote
Microbial Diversity in Marine Environments
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作者:Ali Ebrahimi, Akshit Goyal, and Otto X Cordero
期刊:Elife
在资源分散成颗粒或小区域嵌入到大基质的斑块环境中,微生物觅食在自然环境中发挥着关键作用。在海洋和淡水系统中,与颗粒相关的细菌以不同方式与颗粒表面相互作用:一些只在短期内定殖,而另一些则形成长寿、稳定的菌落。短期和长期定殖共存的生态机制尚未被了解。本文使用数学建模解释了对漂浮颗粒而言不同分离率的海洋种群如何能够稳定地共存。模型中种群只在颗粒上生长,但也面临着由于被捕食和下沉而增加的死亡风险。共存的关键思想是,从粒子分离调节净生长和死亡率,但方向相反,在两者之间创造了一种平衡。虽然缓慢分离的种群表现出最高的增长回报(即产生更多的净后代),但与快速分离的种群相比,它们更容易遭受更高的死亡率。令人惊讶的是,环境的波动,表现为粒子的大量繁殖(有利于生长)和捕食者(有利于死亡)的大量繁殖,大大增加了具有不同分离率的种群共存的可能性。此项研究揭示了海洋微生物的空间生态如何导致可预测的多样化觅食策略,以及多个类群在单一生长限制资源上的共存。
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Figure 4: Schematic representation of the mathematical model simulating slow and fast dispersal strategies of bacterial populations that colonize particulate organic matter (A).
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Microbial foraging in patchy environments, where resources are fragmented into particles or pockets embedded in a large matrix, plays a key role in natural environments. In the oceans and freshwater systems, particle-associated bacteria can interact with particle surfaces in different ways: some colonize only during short transients, while others form long-lived, stable colonies. We do not yet understand the ecological mechanisms by which both short-term and long-term colonizers can coexist. Here, we address this problem with a mathematical model that explains how marine populations with different detachment rates from particles can stably coexist. In our model, populations grow only while on particles, but also face the increased risk of mortality by predation and sinking. Key to coexistence is the idea that detachment from particles modulates both net growth and mortality, but in opposite directions, creating a trade-off between them. While slow-detaching populations show the highest growth return (i.e., produce more net offspring), they are more susceptible to suffer higher rates of mortality than fast-detaching populations. Surprisingly, fluctuating environments, manifesting as blooms of particles (favoring growth) and predators (favoring mortality) significantly expand the likelihood that populations with different detachment rates can coexist. Our study shows how the spatial ecology of microbes in the ocean can lead to a predictable diversification of foraging strategies and the coexistence of multiple taxa on a single growth-limiting resource.
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野火导致热带海洋中浮游植物生物量增加
Wildfires Enhance Phytoplankton Production in Tropical Oceans
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作者:Dongyan Liu, Chongran Zhou, John K. Keesing, Oscar Serrano, Axel Werner, Yin Fang, Yingjun Chen, Pere Masque, Janine Kinloch, Aleksey Sadekov, and Yan Du
期刊:Nature Communications
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全球尺度下,野火的强度和频率大大增加。野火燃烧产生的烟尘中含有大量的营养元素,可通过大气沉降与河流输送进入海洋,但它们对海洋浮游植物生物量的贡献仍所知甚少。本文利用古生态学技术、遥感反演与观测相结合的研究方法,重建了百年来澳大利亚西北部金伯利(Kimberley)海岸野火高发区中野火强度与海洋浮游植物生物量的变化关系。在十年际的尺度上,野火和浮游植物生物量之间存在正相关关系。在印度洋偶极子正相位并伴随强厄尔尼诺的气候背景下,野火对海洋浮游植物生物量的重要性在统计上显著高于热带气旋与降水对其的影响。金伯利沿岸的年代际叶绿素a变化证实了这种现象的空间联系。这项研究结果表明,在预测全球变暖对海洋浮游植物生物量的影响时,必须考虑来自野火的额外营养元素的作用。
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Figure 5: Maps showing changes in the length of the fire weather season on a global scale and sampling location.
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Wildfire magnitude and frequency have greatly escalated on a global scale. Wildfire products rich in biogenic elements can enter the ocean through atmospheric and river inputs, but their contribution to marine phytoplankton production is poorly understood. Here, using geochemical paleo-reconstructions, a century-long relationship between wildfire magnitude and marine phytoplankton production is established in a fire-prone region of Kimberley coast, Australia. A positive correlation is identified between wildfire and phytoplankton production on a decadal scale. The importance of wildfire on marine phytoplankton production is statistically higher than that of tropical cyclones and rainfall, when strong El Niño Southern Oscillation coincides with the positive phase of Indian Ocean Dipole. Interdecadal chlorophyll-a variation along the Kimberley coast validates the spatial connection of this phenomenon. Findings from this study suggest that the role of additional nutrients from wildfires has to be considered when projecting impacts of global warming on marine phytoplankton production.
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全球碳循环之陆地-海洋循环圈
The Land-to-ocean Loops of the Global Carbon Cycle
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作者:Pierre Regnier, Laure Resplandy, Raymond G. Najjar, and Philippe Ciais
期刊:Nature
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海洋和陆地的碳储量通常是单独计算的,并没有充分考虑碳通过内陆水域、河口、潮滩湿地和大陆架水域,即"陆地-海洋水生连续体"(land-to-ocean aquatic continuum, LOAC)从陆地到海洋的输运。本文评估了工业时期之前会受到包括气候变化在内的直接人为干扰的LOAC碳循环。在作者们看来,传统的"远程环路"全球碳循环,即通过河流将碳从陆地生态系统带到开阔海域,会受到两个"短程环路"的加强,这两个"短程环路"指的是从陆地生态系统到内陆水域,从潮滩湿地到开阔大洋。使用质量平衡方法,本文发现在工业化前陆地生态系统吸收大气二氧化碳并输运到海洋或者释放回大气中的碳总量每年为0.65±0.30 pg(±2 sigma)。人类加速了陆地生态系统、内陆水域和大气之间的碳循环,并减少了潮滩湿地和沉水植被对大气二氧化碳的吸收。如果忽视这些不断变化的LOAC碳通量会导致陆地生态系统碳储量每年被高估0.6±0.4 pg,并低估沉积物和海洋碳储量。本文发现的这个知识缺口是减少今后评估LOAC通量不确定性的关键。
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Figure 6: Approaches to quantify the pre-industrial carbon budget.
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Carbon storage by the ocean and by the land is usually quantified separately, and does not fully take into account the land-to-ocean transport of carbon through inland waters, estuaries, tidal wetlands and continental shelf waters—the ‘land-to-ocean aquatic continuum’ (LOAC). Here we assess LOAC carbon cycling before the industrial period and perturbed by direct human interventions, including climate change. In our view of the global carbon cycle, the traditional ‘long-range loop’, which carries carbon from terrestrial ecosystems to the open ocean through rivers, is reinforced by two ‘short-range loops’ that carry carbon from terrestrial ecosystems to inland waters and from tidal wetlands to the open ocean. Using a mass-balance approach, we find that the pre-industrial uptake of atmospheric carbon dioxide by terrestrial ecosystems transferred to the ocean and outgassed back to the atmosphere amounts to 0.65 ± 0.30 petagrams of carbon per year (±2 sigma). Humans have accelerated the cycling of carbon between terrestrial ecosystems, inland waters and the atmosphere, and decreased the uptake of atmospheric carbon dioxide from tidal wetlands and submerged vegetation. Ignoring these changing LOAC carbon fluxes results in an overestimation of carbon storage in terrestrial ecosystems by 0.6 ± 0.4 petagrams of carbon per year, and an underestimation of sedimentary and oceanic carbon storage. We identify knowledge gaps that are key to reduce uncertainties in future assessments of LOAC fluxes.
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对掠食性声音的行为反应可以预测鲸类对恐惧音景中人为噪声的敏感度
Behavioral Responses to Predatory Sounds Predict Sensitivity of Cetaceans to Anthropogenic Noise Within a Soundscape of Fear
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作者:Patrick J. O. Miller, Saana Isojunno, Eilidh Siegal, Frans-Peter A. Lam, Petter H. Kvadsheim, and Charlotte Curé
期刊:Proceedings of the National Academy of Sciences
声波信号可以在海洋环境中有效地传播,使发出响声的捕食者和猎物能够互相窃听。对四种鲸类动物的研究结果表明,它们利用声学信息来评估捕食风险,并且已经进化出通过停止觅食来降低捕食风险的机制。那些在听到虎鲸捕食声音时更容易放弃觅食的物种,在1- 4千赫声纳作用下也更少觅食。这表明那些表现出强烈反捕食反应的物种,对人类噪音也有更强的行为反应。这项在干扰驱动因素的理解上的进展,有助于我们在人类活动日益增加的海洋中,预测水下噪音污染对物种和栖息地的影响程度。
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Figure 7: Representative time series behavioral data recorded by sound-and-movement recording Dtags, with exposure periods marked as boxes. For each species, the Top panels show dive depth versus time, with feeding indicators shown in color (navy blue, echolocation click production; red line, buzz clicks; red crosses, lunges). Bottom panels show the absolute value of vertical speed, with the color indicating the behavioral state. Note the dark-green intense foraging state was associated with feeding indicators and higher vertical speeds. Note the reduction in intense foraging during 1- to 4-kHz sonar treatments (solid boxes) but little effect of the no-sonar control treatment (dashed boxes).
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Acoustic signals travel efficiently in the marine environment, allowing soniferous predators and prey to eavesdrop on each other. Our results with four cetacean species indicate that they use acoustic information to assess predation risk and have evolved mechanisms to reduce predation risk by ceasing foraging. Species that more readily gave up foraging in response to predatory sounds of killer whales also decreased foraging more during 1- to 4-kHz sonar exposures, indicating that species exhibiting costly antipredator responses also have stronger behavioral reactions to anthropogenic noise. This advance in our understanding of the drivers of disturbance helps us to predict what species and habitats are likely to be most severely impacted by underwater noise pollution in oceans undergoing increasing anthropogenic activities.
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甲烷鬃菌与“Candidatus Velamenicoccus Archaeovorus”
Methanosaeta and “Candidatus Velamenicoccus Archaeovorus”
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作者:Jana Kizina, Sebastian F. A. Jordan, Gerrit Alexander Martens, Almud Lonsing, Christina Probian, Androniki Kolovou, Rachel Santarella-Mellwig, Erhard Rhiel, Sten Littmann, Stephanie Markert, Kurt Stüber, Michael Richter, Thomas Schweder, and Jens Harder
期刊:Applied and Environmental Microbiology
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附生细菌可以在细菌细胞上和细胞外生存。本文描述了生活在古细菌和细菌上的超微厌氧附生菌OP3 LiM。作者在缓慢生长的产甲烷菌培养物中检测到丝状古甲烷鬃菌的病死细胞。OP3 LiM以一种糖发酵剂的形式存在,可能依赖于细胞膜外部的多糖,并具有作为互营菌生存的基因组潜力。OP3 LiM的掠夺性生活方式是由其基因组(Candidatus omnitphica门的第一个封闭基因组)和与猎物细胞进行细胞间接触支持的。作者建议将OP3 LiM命名为“Candidatus Velamenicoccus archaeovorus”。其代谢的多功能性解释了“Omnitrophica Candidatus”3在缺氧生境中的普遍存在,并赋予超微附生菌在微生物生物质的循环和再矿化中的重要作用。超微细菌从细胞膜外去除多糖也可能影响原核生物和真核生物之间的生物相互作用。
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Figure 8: LIVE/DEAD staining of a filament assigned to Methanosaeta in images with optimal exposure (A) and overexposure (B) to visualize regions of weak staining. In the DIC micrograph, dead (red) cells had less biovolume than live (green) cells. Overexposure showed a weak green staining of cells and short red cells separating green cells similar to spacer plugs. We assigned the short dead cells to unequally produced daughter cells that precede filament separation of Methanosaeta based on the observation of two spacer plugs between cells visible by Nile red staining (Fig. S8 and S9) and Methanosaeta microcells in thin-section TEM images (Fig. S11, S15, and S16). Bar, 5 μm.
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Epibiotic bacteria are known to live on and off bacterial cells. Here, we describe the ultramicrobacterial anaerobic epibiont OP3 LiM living on Archaea and Bacteria. We detected sick and dead cells of the filamentous archaeon Methanosaeta in slowly growing methanogenic cultures. OP3 LiM lives as a sugar fermenter, likely on polysaccharides from outer membranes, and has the genomic potential to live as a syntroph. The predatory lifestyle of OP3 LiM was supported by its genome, the first closed genome for the phylum “Candidatus Omnitrophica,” and by images of cell-to-cell contact with prey cells. We propose naming OP3 LiM “Candidatus Velamenicoccus archaeovorus.” Its metabolic versatility explains the ubiquitous presence of “Candidatus Omnitrophica” 3 in anoxic habitats and gives ultramicrobacterial epibionts an important role in the recycling and remineralization of microbial biomass. The removal of polysaccharides from outer membranes by ultramicrobacteria may also influence biological interactions between pro- and eukaryotes.
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为什么在复杂的环境管理中少有双赢
Why Win-wins are Rare in Complex Environmental Management
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作者:Margaret Hegwood, Ryan E. Langendorf, and Matthew G. Burgess
期刊:Nature Sustainability
备受瞩目的建模研究通常预测大规模的双赢解决方案是广泛存在的,但现实世界的复杂性,从业者往往对双赢的达成持怀疑态度。本文通过数学方式展示为什么复杂性使双赢难以实现,从而弥合了这一分歧。作者提供了一个普遍的证明,即增加目标数量、利益相关者数量或约束数量会降低双赢结果的可用性(这里指的是帕累托改进)。作者还指出,权衡严重性的度量会增加目标的数量。随着目标的数量接近无穷大,这种权衡严重性的度量会接近一个不受权衡曲面曲率影响的极限。这是令人惊讶的,因为凹面权衡曲面曲率会导致较少目标的较轻严重度的权衡。本文理论表明,随着目标的增加,这种差异会逐渐消失。在元分析中,77% 的经验估计的两目标权衡曲面是凹的。随后,作者展示了如何从较少目标之间的估计权衡开始,将权衡严重度量近似地外推到更多的目标的数量上。本文的结果为建模者提供了精确的直观模型结果,以了解从业者对双赢情景的怀疑,也为从业者解读简单权衡模型提供了指导。
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Figure 9: Tradeoff severity and number of objectives. Panel A shows how the downward-sloping nature of tradeoff surfaces causes X(n) to decrease in n. Panel B illustrates the assumptions of our symmetric tradeoff model, with key parameters α (defining the surface edge) and θ (measuring surface curvature) in two dimensions. Panel C illustrates the difference between convex, concave, and linear tradeoff surface shapes. Panel D shows the gradual convergence of X(n) over a range of tradeoff surface curvatures towards α (α = 0.5 in the examples shown).
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High-profile modelling studies often project that large-scale win–win solutions are widely available, but practitioners are often sceptical of win–win narratives, due to real-world complexity. Here we bridge this divide by showing mathematically why complexity makes win–wins elusive. We provide a general proof that increasing the number of objectives, the number of stakeholders or the number of constraints decreases the availability of win–win outcomes (here meaning Pareto improvements). We also show that a measure of tradeoff severity increases in the number of objectives. As the number of objectives approaches infinity, we show that this tradeoff severity measure approaches a limit unaffected by the curvature of the tradeoff surface. This is surprising because concave tradeoff-surface curvature results in less severe tradeoffs with fewer objectives. Our theory suggests that this difference gradually dissipates as objectives are added. In a meta-analysis, we show that 77% of empirically estimated two-objective tradeoff surfaces are concave. We then show how to approximately extrapolate our tradeoff severity measure to higher numbers of objectives, starting from estimated tradeoffs between fewer objectives. Our results provide modellers with precise intuition into practitioners’ scepticism of win–win narratives and practitioners with guidance for assessing the implications of simple tradeoff models.
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公海条约谈判未能达成协议
High Seas Treaty Talks Fail to Reach a Deal
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联合国国家管辖外海域海洋生物多样性养护与可持续利用协定的第四次政府间谈判会在纽约闭幕,会议未能完成条约谈判的任务。许多国家,包括高雄心联盟的48个成员,承诺在2022年内通过额外会议来完成新条约的签订。
The fourth negotiation session on the legally binding instrument on the Conservation and Sustainable Use of Marine Biodiversity in Marine Areas Beyond National Jurisdiction concluded in New York, having failed to complete its mandate to negotiate the treaty. Many countries, including the 48 members of the High Ambition Coalition, committed to concluding the new treaty through additional meetings during 2022.
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联 系 我 们
IMBeR国际项目办公室(中国)
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IMBeR International Project Office - China
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