MiTeGen Newsletter

Room-Temperature Crystallography Reveals Altered Bindings of Small Molecule Fragments of PTP1B

Room-temperature crystallography reveals

Image Source: Keedy, et al. (2022). Room temperature crystallography reveals altered binding of small molecule fragments to PTP1B bioRxiv 11.02.514751; doi:

In an article at BioRxiv, Daniel A. Keedy et al. perform two RT (room temperature) crystallographic screens, single-crystal and in-situ, of PTP1B using many of the same fragments from an earlier cryo crystallographic screen of PTP1B. This RT screen represents "the largest RT crystallography screens of a diverse library of ligands to date, and enabling a direct interrogation of the effect of data collection temperature on protein-ligand interactions."

"Their main findings suggests that the vast body of existing cryogenic-temperature protein-ligand structures may provide an incomplete picture, and highlights the potential of RT crystallography to help complete this picture by revealing distinct conformational modes of protein-ligand systems. Our results may inspire future use of RT crystallography to interrogate the roles of protein-ligand conformational ensembles in biological function."

Our MiTeGen In Situ-1 crystallography plates were used in this research.

Read The Article At BioRxiv

Cryogenic eCryoTag Sample Tracking System

Cryogenic RFID Sample Tracking System
Cryogenic RFID Sample Tracking System Close Up

After extensive refinement the Cryogenic eCryoTag Sample Tracking System is now available. The system allows you to easily track samples in crystallography and cryo-EM.

How it works - eCryoTags are RFID (Radio-frequency identification) tags that transmit a unique ID. The eCryoTags are embedded into components utilized in sample storage and tracking in cryo-EM and crystallography. These include sample storage pucks, grid boxes, and other components. The eCryoTag ID can easily be read using a simple reading system with a cryogenic compatible wand or via a puck reading station.

Users can correlate the designated ID to their samples thus tracking them. The system is able to be easily integrated into most LIMS.

Learn More About The eCryoTag Sample Tracking System

Did You Know We Offer Phasing Products?

Phasing Products

We offer a range of experimental phasing products for protein crystallographers. These product are heavy-atom kits that are suitable for single/multiple isomorphous replacement (SIR and MIR) experiments and anomalous dispersion (SAD and MAD) experiments.

Two of the most popular phasing products are the Tantalum Cluster Derivatization Kit and JBS Magic Triangle Kit. If you need help in choosing the appropriate phasing product for your research please contact us.

See All Phasing Products
Upcoming Events
At MiTeGen, we sponsor numerous events, conferences, workshops and symposiums throughout the year. These events include fields such as structural biology, crystallography, cryo-EM, beamline research and more. We believe in supporting researchers and our industry. Listed below are two upcoming events you might find interesting.
DLS-CCP4 Data Collection and Structure Solution Workshop

The DLS-CCP4 Data Collection and Structure Solution Workshop is Nov 28th to Dec 6th.  All aspects of structure solution will be covered during the workshop, from data collection through to phasing, refinement and validation. We are sponsoring the workshop.

Learn More
Biophysical Society Annual Meeting 2023

The Annual Biophysical Society meeting is February 18th to 22nd 2023 in San Diego, CA. Includes workshops, poster competition, talks, vendor exhibits and a chance to learn about the newest emerging techniques and applications. We are exhibiting at the meeting

Learn More
Looking For More Events, Workshops and Conferences

If you are looking for more events visit our events page. It lists conferences, workshops, symposiums, user meetings and more.

Topics include structural biology, material science, crystallography, cryo-EM, beamline research and more.

Visit Our Events Page
Industry Resources and News

Here is a small selection of industry resources and news for your browsing.

  • The TamaTA-INNOV program is now open. It provides SMEs with funded access to European light source facilities for their research projects - Learn more  
  • From FEBS Press, A Hitchhiker's guide through the bio-image analysis software universe - Learn more 
  • EMDB has launch Validation Analysis which is a "collection of EMDB resources for validation of cryo-EM structures, focusing on the quality of the volume data alone and that of the fit of any atomic models (themselves archived in the PDB) to the volume data" - Learn more
  • Crystals MDPI is accepting manuscripts for its upcoming special issue, New Advances in Protein Crystallography. Deadline submission is July 31st 2023 - Learn more
  • The European Synchrotron has opened a new serial crystallography beamline named ID29 - Learn more
  • From EMBO reports, AI revolutions in biology. The protein folding model and AlphaFold model limitations - Learn more
  • Update from the CCDC concerning paper mill in crystallography allegations - Learn more
  • The first paper in IUCr's Raw data letters has been published. Crystal structure of the second extracellular domain of human tetraspanin CD9: twinning and diffuse scattering - Learn more
  • Paper at Acta Crystallographica Section D concerning Crystallographic fragment screens, Exiting the tunnel of uncertainty: crystal soak to validated hit - Learn more 
  • Paper at Acta Crystallographica Section D concerning how to improve AlphaFold,  Putting AlphaFold models to work with phenix.process predicted_model and ISOLDE - Learn more 
  • Article at Oxford Academic, RiboXYZ: a comprehensive database for visualizing and analyzing ribosome structures - Learn more 
  • Article at Cell Structure, Assessing PDB macromolecular crystal structure confidence at the individual amino acid residue level - Learn more
  • Summary of the Smart Data Collection Workshop that was this past April at the New York Structural Biology Center with a review of four smart data collection packages - Learn more 
  • Report on Diamond's socio-economic impact on UK science and economy - Learn more
  • From EMPIAR, how to claim your EMPIAR entries to your ORCID profile - Learn more
  • Video tour of the Macromolecular Crystallography Facility at the Indiana University Bloomington (IUB) - Learn more
  • BESSY II has a call for proposals for the 2023-II round with beamtime period of August 2023 to February 2023 - Learn more
  • The European Synchrotron has a call for Long Term Project Proposals. Deadline is January 2023 - Learn more
Industry Jobs
MiTeGen Jobs Board
Searching for a new job? Interested in knowing what positions labs and facilities are offering? Then visit our jobs board on which we list recent industry job offerings.

And remember, if you have an industry relevant job, we are happy to list it on our jobs board.
Visit Our Jobs Board
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