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Analytical and Preparative Size Exclusion Chromatography
Unix™, Zenix®/ Zenix®-C, SRT®/ SRT®-C, and SRT®-10/ SRT®-10C are seven SEC product lines developed based on innovative surface coatings and particle technologies comprised of uniform, hydrophilic, and neutral nanometer thick films chemically bonded on 1.8 µm, 3 µm, 5 µm, or 10 µm high purity and mechanically stabilized silica for UHPLC, HPLC, and FPLC use of Analytical and Preparative Size Exclusion applications.

Utilizing two unique surface coating technologies - stand-up monolayer for Unix™, Zenix®, SRT® and SRT®-10; and lay-down monolayer for Zenix®-C, SRT®-C, and SRT®-10C, on porous silica, Sepax offers optimized surface chemistry choices based on sample type and sample hydrophobicity in order to achieve high resolution performance separation.
Find the Suitable Pore Size
Overlays of Protein Separation on Different Pore Sizes of Zenix 3  µm SEC Columns   

Column: Zenix-SEC (3 µm, 80, 100, 150, 300 Å, 7.8 x 300 mm each);  Mobile phase: 150 mM Phosphate buffer, pH 7.0
Flow rate: 1.0 mL/min; Detector: UV 214 nm; 
Column temperature: 25 C;   Injection volume: 5 µL
 Overlays of Protein Separation on Different Pore Sizes of SRT-C 5  µm SEC Columns 
Column: SRT-C -SEC (5 µm, 300, 500, 1000, 2000 Å, 7.8 x 300 mm each); Mobile phase: 150 mM Phosphate buffer, pH 7.0; Flow rate: 1.0 mL/min; Detector: UV 214 nm; 
Column temperature: 25 C ; Injection volume: 5 µL
Choose the Suitable SEC Surface Chemistry

Sepax offers surface chemistry choices based on sample type and sample hydrophobicity in order to achieve high resolution performance separation. Unix™, Zenix®, SRT® and SRT®-10 use stand-up monolayer bonded on porous silica. Zenix®-C, SRT®-C, and SRT®-10C use lay-down monolayer on porous silica.
Separation of Protein Standards on 
Zenix and Zenix-C SEC-300
Column: Zenix SEC-300, 3µm, 100Å, 7.8×300 mm and Zenix-C SEC-300  3µm, 100Å, 7.8×300 mm;  Mobile Phase: 150mM phosphate buffer, pH 7.0; Flow rate: 1.0 mL/min; Injection Volume: 20 µL; Detector: UV 280 nm; Sample: Proteins thyroglobulin, BSA, Ribonuclease A (2 mg/mL)
Separation of Hydrophobic Monoclonal Antibody F on Zenix and Zenix-C SEC-300

Column: Zenix SEC-300, 3µm, 100Å, 7.8×300 mm and Zenix-C SEC-300  3µm, 100Å, 7.8×300 mm;  Mobile Phase: 150mM phosphate buffer, pH 7.0; Flow rate: 1.0 mL/min; Detection: UV 280 nm; Sample: mAb F 1.23 mg/mL in 10mM sodium succinte, pH 5.0
Unsure which column is best for your work?
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    EOY16-ST729 (Good until 12/31/2016)
     
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