High Resolution MAb IgG1 and IgG2 Charge Variants Separation 
by Sepax Proteomix® Narrow-bore (2.1mm I.D.) SCX column
Sepax Proteomix® Narrow-bore (2.1 mm I.D.) SCX column offers:
        • Ultra-High Resolution
        • High Sensitivity
        • Small sample amount requirement
        • Reduced solvent consumption
Experiment
Vectibix (PanitumumAb) IgG2 Charge Variants Separation - pH Gradient
Sample: 
IgG2 Vectibix (PanitumumAb)
IgG1 Erbitux (Cetuximab)
IgG1 Rituxan (Rituximab)
 
Column: 
Proteomix  SCX NP-5,  5 um, 2.1 x 250 mm PEEK
 
Mobile Phase A 16 mM MES, 10 mM MOPS, 12 mM TAPS, 10 mM CAPSO, 30 mM NaCL pH 5.6  Mobile Phase B : 10 mM MES, 12 mM MOPS, 14 mM TAPS, 16 mM CAPSO, 30 mM NaCL pH 10.2 Sample : Vectibix 10 mg/mL diluted with water from 20 mg/mL stock;  Injection Volume : 1 µL
Erbitux (Cetuximab) IgG1 Charge Variants Separation - pH Gradient
Rituxan (Rituximab) IgG1 Charge Variants Separation  - Salt Gradient vs. pH Gradient
Mobile Phase A:  16 mM MES, 10 mM MOPS, 12 mM TAPS, 10 mM CAPSO, 30 mM NaCL pH 5.6;  Mobile Phase B: 10 mM MES, 12 mM MOPS, 14 mM TAPS, 16 mM CAPSO, 30 mM NaCL pH 10.2 Mobile Phase C: B+1 M NaCl,  Sample: 2 mg/mL Erbitux; Injection Volume: 3 µL
Salt Gradient: Mobile Phase A:10 mM Sodium Phosphate pH 7.5;  Mobile Phase B: A + 1M NaCl;  pH Gradient: Mobile phase A: 16 mM MES, 10 mM MOPS, 12 mM TAPS, 10 mM CAPSO, 30 mM NaCl, pH 5.6;  Mobile phase B: 10 mM MES, 12 mM MOPS, 14 mM TAPS, 16 mM CAPSO, 30 mM NaCl, pH 10.2;  Mobile phase C: B+1 M  NaCl;  Sample : Rituximab 10 mg/mL;  Injection Volume : 1 µL
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