MHC Tetramers for rapid, simple antigen-specific T cell detection
Custom solutions for MHC complex requirements
| MHC Monomers & Tetramers for Antigen Detection
To support the MHC-related research, natural MHC complexes with high biological activity in both monomer and tetramer forms are available. For antigen-specific detection, tetramers provide a more stable binding to the T cell receptor (TCR) for binding studies and FACS. MHC Complexes including (product list here), and peptide-free complexes are also available; all with different fluorescent labels.
The MHC-peptide complex can stably bind to T cell receptors after tetramerization.
MHC Monomers
MHC monomers have low avidity and fast dissociation rates when binding to the TCR.
Can’t find what you need? We offer MHC-peptide complex development servicesusing our HEK293 platform to ensure natural conformation while considering your specific needs and applications.
01 Co-expressed monomer: Biotinylated MHC class I heavy chain and β2-microglobulin（β2m）are co-expressed in HEK293 systems to get the MHC class I monomer(peptide free) by purified in vitro.
02 Short peptide loading: Monomer MHC class I-peptide complex was obtained by co-incubation of MHC class I (peptide free) monomer with antigenic peptide.
03 Tetramerization: Subsequently, MHC class I-peptide complex monomers are linked by the addition of fluorochrome-labeled streptavidin.
