TSA (Trypticase Soy Agar)
Trypticase Soy Agar (TSA) is primarily used for the isolation and cultivation of a wide variety of microorganisms. It is the same culture media as described in USP Chapter <61> as Soybean Casein Digest Agar Medium (SCD). TSA is also used as a base for blood agar media.
TSA is widely used for maintaining clinical laboratory microorganism stock cultures, plate counting, isolation and enumeration of microorganisms for samples from a variety of sources including cleanroom air, surface and glove samples, water and wastewater samples, cosmetic products, dairy products, and food products.
TSA is highly nutritious and non-selective, allowing growth of fastidious and non-fastidious microorganisms; including many pathogens. Always refer to appropriate procedures and methods for sample collection, inoculation, and disposal, and perform biochemical or serological testing to identify organisms of interest as necessary.