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We are delighted to introduce Tocris Bioscience's latest fluorescent dyes Janelia Fluor™ 549, SE and Janelia Fluor™ 646, SE, developed by Luke Lavis' lab at the Janelia Research Campus and licenced from the Howard Hughes Medical Institute (HHMI).

Janelia Fluor™ dyes are bright, photostable and cell permeable. They have been successfully used in super resolution microscopy, including live cell dSTORM.

As the focus of a ground-breaking article in the journal Nature Methods (cited over 110 times since March 2015) Janelia Fluor™ dyes are an exciting new tool for life science research. 
Key Features
  • Cell Permeable
  • Bright and Photostable
  • Rhodamine-based
  • Available as NHS ester
Applications
  • Super resolution microscopy, including dSTORM in live and fixed cells
  • Single molecule tracking in live cells
  • Confocal fluorescent imaging
  • Suitable for multiplexing
Created using Janelia Fluor™ dyes, this single-cell image is an overlay of a diffusion map of Tetracycline Repressor Protein (green), which leads to antibiotic resistance, and a super-resolution image of histone H2B (purple). 
See how Janelia Fluor™ dyes have been used in research

CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells

Deng et al. (2015) Proc Natl Acad Sci U.S.A 112, 11870. PMID 26324940

 

Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals Halstead et al. (2015) Science 347, 1367. PMID 25792328

 

Dynamics of CRISPR-Cas9 genome interrogation in living cells

Knight et al. (2015) Science 350, 823. PMID 26564855

 

Real-time imaging of Huntingtin aggregates diverting target search and gene transcription

Li et al. (2016) eLife 5, e17056. PMID 27484239

 

Real-time quantification of single RNA translation dynamics in living cells

Morisaki et al. (2016) Science 352, 1425. PMID 27313040

 

Increased spatiotemporal resolution reveals highly dynamic dense tubular matrices in the peripheral ER Nixon-Abell et al. (2016) Science 354, pii: aaf3928. PMID 27789813

 

Live Cell Imaging Reveals the Dynamics of Telomerase Recruitment to Telomeres

Schmidt et al. (2016) Cell 166, 1188. PMID 27523609

 

Single-molecule studies of origin licensing reveal mechanisms ensuring bidirectional helicase loading Ticau et al. (2015) Cell 161, 513. PMID 25892223

 

Visualizing the dynamic coupling of claudin strands to the actin cytoskeleton through ZO-1

Van Itallie et al. (2016) Mol Biol Cell PMID 27974639

 

Glutamate-induced RNA localization and translation in neurons

Yoon et al. (2016) Proc Natl Acad Sci U.S.A. 113, E6877. PMID 27791158




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