Improving Crystallization On Earth and In Space!
Today @ 4:30 PM - In Situ-1™ Plates Launch Into Space!
While our crystallization plates were designed for earth-based crystallization, it turns out the unique features we developed also make them great for growing crystals in micro-gravity. This is the second time these plates will be leaving earth for experiments aboard the International Space Station, having already flown on SpaceX CRS-8. We are very excited about the launch. We will be watching it live and you can too!
The Launch:
Follow SpaceX CRS-14 Launch Coverage Today:
The SpaceX launch of the 14th Commercial Resupply Services (CRS-14) mission is scheduled to launch on April 2nd, 2018 at 4:30 PM ET. This launch to the space station will include a selection of MiTeGen crystallization platforms in the Protein Crystal Growth Kit (see image of actual kit below). These experiments will research the impact that micro-gravity has on crystal growth and obtaining high-quality crystals.

Catch all the excitement of pre-launch and launch updates today starting at 4:00 p.m. EDT on NASA TV!
The Plates:
In Situ-1™ Crystallization Plates: Great for Space, Even Better In Your Lab
Improve Crystal Growth & Reproducibility

• Controlled vapor diffusion via micro-channels provides stable nucleation and growth

Produce Results in Your Lab with Versatility

• Reservoir retention ledge allows for transport and inversion for easy hanging drops

Enhanced Screening & Data Collection:

• Ultra thin-film backing allows in-plate X-ray diffraction
The Payload:
Regular and Modular In Situ-1™ Crystallization Plates
Below is an image of the actual Protein Crystal Growth Kit being sent up today. The photo is courtesy of Kristofer Gonzalez-DeWhitt. Next month, we will be featuring a spotlight on the research that Kris is doing on the ISS.
(Image Contents - Top-left: MiTeGen Modular In Situ™ Plates (G2), Top-right: Regular In Situ™-1 plates)
Looking for better crystals? Try the latest screens!
XP Screen (Protein Crystal Glue)
(Figure adapted from [1], used by courtesy of Prof. Annette Rompel, University of Vienna, Austria)
A crystallization screen for improved crystal quality and phasing
The XP screen provides 96 of the most prominent crystallization conditions upgraded with "Protein Crystal Glue" a universal and flexible crystallization additive known as Anderson−Evans polyoxotungstate [TeW 6 O 24 ] 6−  (TEW) This additive promotes protein crystallization, even for the most challenging targets, and often improves the diffraction quality of protein crystals.
TEW was shown to:
  • Covalently bind and structurally adapt to fit into protein molecules (cgAUS1, PDB code: 4Z12, 4Z13)[2]

  • Act as a linker in various orientations and thereby create either smaller (abPPO4, PDB code: 4OUA) or larger (lysozyme, PDB code: 4PHI) protein-protein distances [3,4]

  • Induce heterogeneous crystallization of two different protein forms in one single crystal (abPPO4, PDB code: 4OUA)[4]
[1] Bijelic et al. (2017) Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography. Acc. Chem. Res. 50:1441. 
[2] Molitor et al. (2016) In situ formation of the first proteinogenically functionalized [TeW6O24O2(Glu)]7- structure reveals unprecedented chemical and geometrical features of the Anderson-type cluster. Chem. Commun. 52:12286. 
[3] Bijelic et al. (2015) Hen Egg-White Lysozyme Crystallisation: Protein Stacking and Structure Stability Enhanced by a Tellurium(VI)-Centred Polyoxotungstate. ChemBioChem 16:233. 
[4] Mauracher et al. (2014) Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal. Acta Cryst. D 70:2301.
The Berkeley Screen

Having a difficult time getting hits?

This screen has been proven to provide crystals [1] . It was developed using a statistical analysis of the Biological Macromolecular Crystallization Database, by researchers at the Berkeley Center for Structural Biology at the Advanced Light Source of Lawrence Berkeley National Laboratory .

These conditions have been extensively used to crystallize target proteins from the Joint BioEnergy Institute and the Collaborative Crystallography program at the Berkeley Center for Structural Biology.

Universal Pucks (Uni-pucks) and Tools
Proven quality and dependability
Planning to collect data remotely? Don't risk losing all of your hard work.

Ship your crystals confidently by obtaining Universal Pucks (Uni-pucks) and Tools from the leading puck supplier in crystallography.

Our products have been used to transports thousands and thousands of crystals to synchrotrons all around the world.

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Learn How Cryocooling Rates Impact Data
NANUQ™ Cryocooling Technology provides reproducible plunge cooling up to 10x faster than hand-plunging techniques. Faster cooling means less time for the nucleation and growth of ice crystals and better data.
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  • Eliminate ice issues
  • Reduce dependency on cryoprotectants
  • Improve crystal diffraction
  • Reduce mosaicity
  • Prevent damage with automated puck filling
Get the info every crystallography lab needs:
All crystallography labs need access to the latest technology. Enable your lab by requesting a free printed catalog of the most advanced tools for crystallization, crystal harvesting and Cryo-EM.
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