SARSCoV2 Main Protease and Multi-temperature X-ray Crystallography | |
Image Source: Keedy, et al. (2022). The temperature-dependent conformational ensemble of SARS-CoV-2 main protease (Mpro). IUCrJ, volume 9, Part 5 https://doi.org/10.1107/S2052252522007497 | |
In a paper at IUCrJ by Daniel A. Keedy, et al., the SARSCoV2 main protease is examined using multi-temperature X-ray crystallography with temperatures ranging from cryogenic to room temperature as well as at high humidity.
Using this approach, the researchers "discovered subtle structural shifts that offer clues about how the enzyme works." 1 In addition, the findings from the research "may inspire the design of new drugs to counteract COVID-19—and possibly help head off future coronavirus pandemics." 2 (1 & 2 Brookhaven National Laboratory, 2022, Phys.org, First structure of key COVID enzyme at human body temperature)
Our MiTeGen MicroMesh loops and MicroRT capillaries were used in this research.
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New Cryo-EM Grid Clipping Tools | | |
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The new grid clipping tool set for cryo-EM consists of 3 components which are the clipping tool, C-clip bottoming tool and the clipping tool rack.
The clipping tool is designed to make the loading of C-clips and clips grids easier and helps avoid frost from environmental moisture. The C-clip bottoming tool provides an easier and more efficient way to “bottom” the C-clip at the tip of the barrel of a clipping tool prior to clipping. The rack holds 4 clipping tools at once which makes your workflow smoother.
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NVH Oil for Room and Variable-Temperature Diffraction Measurements | | |
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Have you tried our NVH oil for your room and variable-temperature diffraction measurements? NVH oil is a very high viscosity oil that minimizes crystal motion during data collection, even when thick layers are used to prevent dehydration.
NVH oil has been used in numerous research projects and is trusted by researchers. See a sampling of these projects via Google Scholar.
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At MiTeGen, we sponsor numerous events, conferences, workshops and symposiums throughout the year. These events include fields such as structural biology, crystallography, cryo-EM, beamline research and more. We believe in supporting researchers and our industry. Listed below are two upcoming events you might find interesting. | |
Registration for the 26th Congress & General Assembly of the Inter-national Union of Crystallography is now open. It is being held August 22-29, 2023, in Melbourne Australia. Meet and hear from other members of the crystallographic community. We are sponsoring and exhibiting at IUCr 2023. | |  |
2023 is fast approaching and the Biophysical Society cryo-EM subgroup will be meeting February 18th, 2023, in San Diego, California during the 67th Annual Meeting of the Biophysical Society. Come hear talks, meet other cryo-EM practitioners and see new cryo-EM innovations. We are a sponsor of the subgroup. | | |
Looking For More Events, Workshops and Conferences | | |
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If you are looking for more events visit our events page. It lists conferences, workshops, symposiums, user meetings and more.
Topics include structural biology, material science, crystallography, cryo-EM, beamline research and more.
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Industry Resources and News | |
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Here is a small selection of industry resources and news for your browsing.
- ThermoFisher Scientific is offering a series of in person events designed to get you started in cryo-EM. These events are being held across the United States - Learn More
- MDPI Crystals journal is accepting submissions for its upcoming special issue, "X-ray Crystallography and Drug Discovery". Deadline for submission is March 15th, 2023 - Learn More
- COSMIC2 has released a new tool named OmegaFold. It is a protein structure prediction algorithm that uses a protein language model to predict structure - Learn More
- COSMIC2 has created a brief primer comparing the five different prediction tools to help you determine which one to use - Learn More
- Cambridge Crystallographic Data Centre (CCDC) has announced a "new grant scheme to grow the scientific community and engage students to study STEM. Grants cover costs to produce resources or activities that will increase schools and public engagement in crystallography and structural science." - Learn More
- Cambridge Crystallographic Data Centre (CCDC) shared "a slide pack from Frank Schaper which gives a short, succinct, and visual intro to crystallography including unit cells, space groups, and diffraction experiments." This slide pack is meant to be used in teaching undergraduate chemistry - Learn More
- ResearchGate and the International Union of Crystallography (IUCr) have established a content partnership "with the goal of facilitating the discovery of and access to new research in crystallography. Authors submitting to these journals will see their articles added automatically to their publication pages on ResearchGate." - Learn More
- Article at Nature, MeasureIce: accessible on-the-fly measurement of ice thickness in cryo-electron microscopy - Learn More
- Article at eLife Sciences concerning Smartscope which is an automated systematic evaluation of cryo-EM specimens - Learn More
- Paper at Acta Crystallographica Section D, CCP4 Cloud for structure determination and project management in macromolecular crystallography - Learn More
- Paper at Acta Crystallographica Section D, ModelCraft builds more complete models than the Buccaneer pipeline in CCP4i, especially when starting from poor or partial molecular-replacement solutions - Learn More
- Instruct-ERIC is soliciting users for its centers offering cryo-EM, NMR and X-ray techniques - Learn More
- Diamond Light Source's eBIC4i facility is offering remote access to its cryo-EM instruments - Learn More
- CERIC has a call for proposals for access to 50+ labs and techniques in its integrated multidisciplinary facilities for materials and biomaterials - Learn More
- PAL-XFEL has a call for protein crystal screening proposals. Deadline is September 26th - Learn More
- APS is accepting proposals for the 2023-1 beamtime run. Deadline is October 28th - Learn More
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Searching for a new job? Interested in knowing what positions labs and facilities are offering? Then visit our jobs board on which we list recent industry job offerings.
And remember, if you have an industry relevant job, we are happy to list it on our jobs board.
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