Editor's Note
The authors of this study established a quick, simple and accurate method to determine the components of ferulic acid, coniferyl ferulate and ligustilide in  Angelicae Sinensis  Radix. No significant difference of results was found between 1H-qNMR method established by the authors and HPLC-UV in determining the content of three components in three batches  Angelicae Sinensis Radix. The study concludes that the method can be used for simultaneous determination of three active components, and provide a scientific basis for the overall quality evaluation and quality control of  Angelicae Sinensis  Radix.
Objective

In this study, a quick, simple and accurate method was established to determine the components of ferulic acid, coniferyl ferulate and ligustilide in Angelicae Sinensis  Radix.

Methods

Using dimethyl sulfoxide-d 6 (DMSO-d 6 ) as the test solvent and pyrazine as the internal standard substance, 1H-qNMR measurement was performed on a 600MHz spectrometer. The quantitative resonance peaks of pyrazine, ferulic acid, ligustilide, and coniferyl ferulate were at δ 8.66 ppm, δ 6.35-6.37 ppm, δ 5.53-5.55 ppm, δ 6.50-6.53 ppm, respectively.

Results

The linear relationship, limit of detection, limit of quantification, precision, stability and recovery were verified and the results were good. On the other hand, it was verified by HPLC, and the HPLC used for verification passed the methodological investigation of linearity, precision, repeatability, stability and recovery, and the results were good. In addition, no significant difference of results was found between 1H-qNMR method and HPLC-UV in determining the content of 3 components in 3 batches  Angelicae Sinensis  Radix.

Conclusion

The method can be used for simultaneous determination of 3 active components, and providing a scientific basis for the overall quality evaluation and quality control of  Angelicae Sinensis  Radix.

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