Editor's Note
This study evaluated the protective effects of water extracts of hemp flowers in a model of ulcerative colitis. Extracts from four commercial hemp cultivars were characterized (Futura 75, Kc virtus, Carmagnola Cs and Villanova), and all tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. The results support the potential efficacy of hemp inflorescence water extracts in managing the clinical symptoms related to ulcerative colitis.
Highlights
  • Hemp inflorescence water extract as innovative sources of anti-oxidant compounds.
  • Biocompatibility of hemp inflorescence water extract in multiple toxicological models.
  • Protective effects of hemp inflorescence water extract in experimental IBD, ex vivo.

Abstract
One of the most promising economic perspectives of hemp production chain is female inflorescence valorization, despite there being actually no chemical composition or biological data from water fraction. In this context, the focus of this study is the evaluation of protective effects related to hemp water  flower extracts from four commercial  cultivars  (Futura 75, Kc virtus, Carmagnola  Cs  and Villanova). We evaluated the  phytochemical  profile through validated spectrophotometric and HPLC methods. Then, we studied the biological activity on C2C12 and HCT116 cell lines , and in an  ex vivo experimental model of ulcerative colitis , constituted by isolated LPS-stimulated colon. Particularly, we assayed the blunting effects induced by hemp water extract treatment on LPS-induced levels of nitrites , malondialdehyde (MDA), prostaglandin (PG)E 2 and serotonin (5-HT). All tested cultivars displayed similar total phenolic and flavonoid profile. However, Futura 75 water extract displayed a better antioxidant and anti-inflammatory profile. Considering this, Futura 75 extract activity has been subsequently assayed on bacterial and fungal species involved in ulcerative colitis, finding a significant inhibition on  C. albicans and selected Gram positive and negative bacterial strains.

Concluding, our results support the potential efficacy of hemp inflorescence water extracts in managing the  clinical symptoms  related to ulcerative colitis.