- Shade treatment reduced the contents of most catechins in preharvest tea leaves.
- Shade treatment increased the theaflavin contents in preharvest tea leaves.
- Shade treatment increased polyphenoloxidase (PPO) activity in preharvest tea leaves.
- CsPPO3 was highly expressed under shade treatment.
- CsPPO3 recombinant protein exhibited PPO function.
Catechins and theaflavins are important metabolites contributing to tea function and quality. Catechins are known to transform into theaflavins during the tea manufacturing process, but the same transformation in preharvest tea leaves is unknown. Herein, we determined that shade treatment (dark), an agronomic practise widely used in tea cultivation, reduced the contents of most catechins, but increased the theaflavin contents, in preharvest tea leaves (cv. Yinghong No.9). This was attributed to the activation of polyphenoloxidase (PPO) activity in darkness. Furthermore,
was highly expressed under darkness, and thus
had been cloned, sequenced, and characterization. The
recombinant protein exhibited PPO function. Furthermore, shade treatment also reduced the catechin contents and increased the theaflavin contents in Yabukita and Hoshinomidori, suggesting that this phenomenon might not be specific to certain tea cultivars. This information will aid in understanding of theaflavin formation and its response to environmental factors at the preharvest tea stage.