Three species of widow spiders are native to Florida, and a fourth species has been introduced. No species of recluse spiders are native to Florida, but three species have been intercepted and occasionally have established populations in single buildings at scattered locations.

Both types of spiders tend to be found in similar places, which is in or under objects where their presence is not necessarily obvious. In the interest of safety, it is recommended that people engaged in activities in which they cannot see where their hands are being placed (such as lifting boards or firewood, or reaching into storage boxes) should wear gloves to prevent being bitten by a hidden spider.