Targeting TrkA: Biosensis TrkA Antibodies in Action

Tyrosine Kinase Receptor A (TrkA) is a high-affinity receptor for nerve growth factor (NGF) and plays a critical role in the development, function, and survival of the nervous system. As a member of the tropomyosin receptor kinase (Trk) family, TrkA is predominantly expressed in sensory neurons, sympathetic neurons, and certain central nervous system (CNS) populations. Its activation initiates downstream signaling cascades—such as the MAPK/ERK, PI3K/AKT, and PLC-γ pathways—that regulate cell differentiation, neuroprotection, and synaptic plasticity.

TrkA-mediated signaling plays a vital role in neurobiology, supporting neuronal survival during development, promoting axonal outgrowth and guidance, regulating synaptic modulation, mediating pain signaling in peripheral sensory neurons, and providing neuroprotection under pathological conditions. Due to its central role in these critical processes, TrkA has emerged as a key molecular target in a range of neurological and neurodegenerative disorders, including Alzheimer’s disease, Parkinson’s disease, chronic pain syndromes, and peripheral neuropathies.

Biosensis Antibodies targeting TrkA provide precise detection and modulation of TrkA signaling. These antibodies are invaluable in studying neurodevelopment, disease progression, and potential interventions across a wide range of neurological disorders. Our antibodies are optimized for IHC/ICC, IF, Western Blot, ELISA, and Flow Cytometry.

Mouse Monoclonal Antibody to TrkA (intracellular Domain)- M-1719-100

The Mouse Monoclonal Antibody to TrkA is specifically designed to target the intracellular domain of human TrkA. This antibody is ideal for use in Western blots to detect full-length and enzymatically cleaved TrkA fragments. It is also valuable for immunocytochemistry of TrkA where other internal marker proteins are also being detected, as the M-1719-100 intracellular epitope requires permeabilization of the cell membranes to be the most effective.

Mouse Monoclonal Antibody to TrkA (Extracellular Domain)- M-1723-100

The Mouse Monoclonal Antibody to TrkA is specifically designed to target the extracellular domain of human TrkA. This antibody is ideal for use in flow cytometry applications, allowing researchers to detect and analyze TrkA expression on the surface of live, non-permeabilized cells. It has been thoroughly validated on SH-SY5Y neuroblastoma and DU145 prostate cancer cell lines, showing strong and specific binding to the extracellular domain of TrkA. This makes it an ideal choice for studies in neurobiology and cancer research that require accurate flow cytometric analysis of TrkA expression.

Rabbit Polyclonal Antibody to TrkA (Whole Serum)- R-152-100

The Rabbit Polyclonal Antibody to TrkA offers a broad reactivity to TrkA, detecting multiple epitopes on the protein. It is well-suited for immunohistochemistry (IHC) on frozen tissue sections, as well as for ELISA applications, where it can be used to detect and quantify TrkA levels. This antibody is an excellent tool for researchers investigating TrkA expression in tissue samples, particularly in the context of neurodegenerative diseases or developmental studies. Interestingly, it also works excellently in non-reduced Western Blots and immunoprecipitation applications, and being a rabbit hosted antibody allows easy access to these unique applications.

Tyrosine Kinase Receptor A (TrkA), Rabbit polyclonal Antibody (R-152-100)

Immunohistochemical staining of Tyrosine Kinase Receptor A (TrkA) in rat spinal cord (free-floating cryostat section, Nickel enhanced DAB IHC) using rabbit antibody (R-152-100) at a dilution of 1 in 2000. Courtesy of Professor Xin Fu Zhou, The Flinders University of South Australia.

Western blot of TrkA receptor in human neuroblastoma SH-SY5Y cell lysate (30 µg/lane). R-152-100 detects TrkA full-length protein at 130-140 kDa under non-reducing conditions (no DTT or beta-mercaptoethanol!). 

Tyrosine Kinase Receptor A (TrkA), Mouse Monoclonal Antibody (M-1723-100)

TrkA expression on non-permeabilized human SHSY-5Y neuroblastoma and DU145 prostate cancer cell lines was analysed by Flow Cytometry with M-1723-100 antibody. Secondary antibody: Goat anti-mouse PE (1:100 dilution, 20 min in the dark on ice. Non-specific Control IgG, clone X63 (cat# M-1249-100) was used as a negative control under the same conditions (black dashed).

Immunofluorescence analysis of TrkA expression on human SHSY-5Y neuroblastoma cell membrane with M-1723-100. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 µg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). Magnification: 100x.

Tyrosine Kinase Receptor A (TrkA), Mouse Monoclonal Antibody (M-1719-100)

Western blot of TrkA in rat PC12 cell lysates using M-1719-100. M-1719-100 detects membrane-associated TrkA full-length protein at approximately 140 kDa (gp140TrkA) as well as the intracellular glycosylated immature form of TrkA at 110 kDa (gp140TrkA), and an intracellular cleavage product at 41 kDa (p41, see Cabrera et al., 1995; Rossi et al., 2002). See the webpage for more details.

Immunofluorescence analysis of TrkA expression in human DU145 prostate cancer cells with M-1719-100 anti-TrkA monoclonal. TrkA antibody M-1719-100 binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 µg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). Magnification: 100x.