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The Complement System, An Innate Immunity Station

The Complement System occupies a unique position among early cellular sensors, acting directly on the surface of triggering cells or substances to coordinate downstream cellular humoral immune responses. This makes this system a critical part of the immune cascade, contributing to both innate immune responses, including:

  • Local inflammation for removal and killing of pathogens
  • Signaling and influencing of T and B-cell biology

As a global regulator of immunity and tissue homeostasis, the Complement System has become an interesting, yet complex pharmacological target, contributing a network that contributes to pathogenesis in various inflammatory and neurodegenerative diseases, as well as, cancers. 

 Anti-complement drugs currently in clinical development

A better understanding of the complement's structure, function, and the biological role will support our complement-based understanding to contribute to treating inflammation, neurodegenerative diseases, and cancers. Complement-targeted drugs that intervene at the signaling cascade level can help to better tailor therapeutic strategies and allow such strategies to be applied to a wider range of indications. To assist with research into the Complement system, ACROBiosystems has developed a series of recombinant complement proteins expressed by HEK293 Cells, including C2, C3, C5, C5a, and Complement factor D (CFD).

Verification Data

High bioactivity verified by antibody binding

Immobilized Human Complement C5, His Tag (Cat. No. CO5-H52Ha) at 2 μg/mL (100 μL/well) can bind Eculizumab Biosimilar with a linear range of 0.1-4 ng/mL (Routinely tested).

Protocol

Immobilized Human Complement C5a, His Tag (Cat. No. C5A-H51H9) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-4 ng/ml (QC tested).

Protocol

High bioactivity verified by cell-based assay

Human Complement C5a, Tag Free (Cat. No. C5A-H5116) induce N-acetyl-β-D-glucosaminidase release from differentiated U937 cells. The ED50 for this effect is 0.215-0.323 μg/mL (Routinely tested).

Protocol

>> Learn More about Promising Cytokine Targets

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