Science Behind Why Apples Turn Brown
Apples contain an enzyme called polyphenol oxidase (PPO). When you slice open an apple, the PPO reacts with the oxygen in the air and enzymatic browning occurs. The chemical reaction between the PPO and oxygen produces melanin, which is what gives the apple flesh the brown color.
Since lemon juice worked so well, we at first thought acidic liquids, or liquids with a low pH level, would prevent the apple from browning. However, vinegar has a low pH level as well and it seemed to have accelerated the browning. So either we made an error in the experiment, or something else is at play here.
Lemon juice contains ascorbic acid or Vitamin C. Ascorbic acid will react with the oxygen before the oxygen reacts with the enzyme in the apple. Therefore, ascorbic acid will prevent the apple from browning until all the acid is used up. Vinegar does not have ascorbic acid, and therefore it did not work.
It is inconclusive why vinegar seemed to have sped up the browning. Vinegar is essentially acetic acid and water. Perhaps we need to dilute the vinegar with water? Is pure white vinegar too strong and started deteriorating the apple flesh? That’s an experiment for another day.
As for baking soda and milk, they are on the other end of the pH spectrum since they are bases.
Interestingly, milk did fairly well in preventing the apple from oxidizing. A future experiment can be designed to see if the fat content in the milk matters when it comes to blocking oxygen from reacting with the enzymes in the apple.
Baking soda, however, made the apple brown faster. Alkaline (high pH level) liquids contain higher amounts of oxygen. Therefore, covering the apple with baking soda solution actually brought more oxygen to the surface of the apple, accelerating the browning.
Salt water prevented the oxidation of the apple slice because it surrounded the apple cells with a saltier environment than the inside of the cells. As a result, water moves outside of the cells through osmosis in an attempt to equalize the concentration of salt and water inside and outside of the cell.
The water and salt on the surface of the apple slice interfere with oxygen reaching the fruit’s surface and therefore slowing down oxidation.