The RNA Transcript, April 19, 2021
TODAY, Monday, April 19, 4:00 pm ET | U-M Center for RNA Biomedicine, RNA Innovation Seminar Series

“Structural insights into mRNA translation initiation in humans”
Jailson (Jay) Brito Querido, Ph.D., Postdoctoral Scientist, MRC Laboratory of Molecular Biology, Cambridge, UK

Dr. Querido was featured in the Member Spotlight Series of The RNA Society.
To better understand how RNA in bacteria gives rise to protein—and along the way, target these processes in the design of new antibiotics—researchers are turning their attention to the unique way this process happens in bacteria. 

The new results promise to have important implications for the future design of antibiotics that could target the coupling mechanism instead of targeting the transcription or translation processes separately.

A PNAS (April 2021) publication by Center for RNA Biomedicine members and collaborators Surajit Chatterjee, Adrien Chauvier, Shiba S. Dandpat, Irina Artsimovitch and Nils G. Walter.

Figure caption: The 30S subunit of the ribosome can dynamically bind to the nascent mRNA as soon as its binding site emerges from the RNAP. Transcription factors and translation initiation factors assist in the initial binding and retention of the 30S subunit on the mRNA, resulting in the stabilization of an early initiation complex. During translation, the ribosome can follow the leading RNAP, establishing transcription-translation coupling and maintaining optimal transcription speed. In the presence of the ligand preQ1, transcription-translation coupling is disrupted, surprisingly leading to slow transcription.

Paper cited: A translational riboswitch coordinates nascent transcription-translation coupling, Surajit Chatterjee, Adrien Chauvier, Shiba S. Dandpat, Irina Artsimovitch and Nils G. Walter, PNAS April 20, 2021 118 (16) e2023426118; https://doi.org/10.1073/pnas.2023426118
Tuesday, April 20, 3:00 pm EST | U-M CMB Short course
ZOOM meeting ID: Meeting ID: 945 0928 0988

Cari Vanderpool, Ph.D., University of Illinois
Wednesday, April 21, 9:00–10:00 am ET | RNA Collaborative Seminar Series, hosted by Shanghai RNA Club

“Structural insights into molecular mechanism for N6-adenosine methylation by MT-A70 family methyltransferase METTL4”
Jinbiao Ma, Ph.D., Institute of Plant Biology, Fudan University

“A fight against SARS-CoV-2 powered by RNA structure systems biology and machine intelligence”
Qiangfeng Zhang, Ph.D., School of Life Sciences, Tsinghua University

Friday, April 23, 10:00–11:00 am ET | U-M Life Sciences Institute, U-M Structure Seminar

"Structure and substrate selection of Saccharomyces cerevisiae pseudouridine synthase 7"
Meredith Purchal, Graduate Student, Koutmos Lab, University of Michigan

Read M. Purchal's U-M Center for RNA Biomedicine "featured scientist" profile.
Monday, May 3, 4:00–5:00 pm ET | U-M Center for RNA Biomedicine

“Regulating the Regulators: Control of RNA Binding Proteins during Embryogenesis”
Olivia Rissland, Ph.D., Assistant Professor, RNA Bioscience Initiative, Department of Biochemistry & Molecular Genetics, University of Colorado School of Medicine

 
Contact Elisabeth Paymal for press releases and blog articles of your upcoming top journal publications.

Our members' publications are available through Altmetric. Five queries are currently available: "RNA," "microRNA," "Transcriptome," "Translation," and "Molecule." Please make sure to have at least one of these key words in your title or abstract. Below are recent highlights.
A translational riboswitch coordinates nascent transcription-translation coupling, Surajit Chatterjee, Adrien Chauvier, Shiba S. Dandpat, Irina Artsimovitch and Nils G. Walter, PNAS April 20, 2021 118 (16) e2023426118; https://doi.org/10.1073/pnas.2023426118


Abstract: Bacterial messenger RNA (mRNA) synthesis by RNA polymerase (RNAP) and first-round translation by the ribosome are often coupled to regulate gene expression, yet how coupling is established and maintained is ill understood. ... A model emerges wherein mRNA structure and transcription factors coordinate to dynamically modulate the efficiency of transcription–translation coupling.
A PRC2-independent function for EZH2 in regulating rRNA 2′-O methylation and IRES-dependent translation, Yi, Y., Li, Y., Meng, Q. et alNat Cell Biol 23, 341–354 (April 2021). https://doi.org/10.1038/s41556-021-00653-6

Abstract: Dysregulated translation is a common feature of cancer. Uncovering its governing factors and underlying mechanism are important for cancer therapy. .... Our findings reveal a previously unrecognized role of zeste homologue 2 (EZH2) in cancer-related translational regulation.
For Fun
By the end of 2021, how many kilograms of RNA will Moderna Therapeutics have made?
(answer next week!)
13 kg
67 kg
128 kg
140 kg
LAST WEEK QUESTION:
The precise study of color as generalized from visible light to all bands of the electromagnetic spectrum is called...

ANSWER: Spectroscopy. Two types for spectroscopy techniques are performed at the SMART Center, one of the U-M Center for RNA Biomedicine's core facilities: Fluorescence correlation spectroscopy and force spectroscopy.

More information about the SMART Center and the various techniques for single molecule analysis in real-time.
Sethu Pitchiaya, Ph.D., at the SMART Center
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