The RNA Transcript, July 6, 2020
We're pleased to resume our
RNA Innovation Seminar Series
July 13, 4:00–5:00 pm
The University of Michigan Center for RNA Biomedicine regroups over 150 faculty members and their labs across seven Schools and Colleges on the Ann Arbor campus. We're pleased to feature these outstanding scientists and colleagues in our weekly news.
Clinical Lecturer, Pediatric/Oncology
Tuesday, July 7, 2020, 4:00 pm | Taubman Tech Talk

"Next Generation Sequencing: The Long and the Short of it"
Olivia Koues, Ph.D., Managing Director, Advanced Genomics Core
Friday, July 10, 2020, 11:00 am | MD Anderson Cancer Center

"Challenges and Strategies in Ascribing Functions to Long Noncoding RNAs in Normal Physiology and Cancer"
Monday, July 13, 2020, 4:00 pm | U -M Center for RNA Biomedicine

"lnc’ing RNA to Innate Immunity"
Kate Fitzgerald, UMassMed , Vice Chair, Research, Department of Medicine,Director, Program in Innate Immunity, Worcester Foundation Chair in Biomedical Sciences, Department of Medicine, University of Massachusetts Medical School
Wednesday, July 15, 2020, 4:00–5:00 pm | RNA Collaborative Seminar Series

"Programmable RNA-targeting by CRISPR-Cas enzymes”

“Coupled protein synthesis and ribosome-guided piRNA processing on mRNAs”

Moderator: Lynne Maquat, Ph.D.

Our members' publications are now available through Altmetric. Six queries are currently available: "RNA," "microRNA," "Transcriptome," "Translation," and "Molecule." Please make sure to have at least one of these key words in your title or abstract. Below are the highlights from the last four weeks.
Coupling of translation quality control and mRNA targeting to stress granules

Using single mRNA imaging, we discovered ribosomes stall on some mRNAs during arsenite stress, and the release of transcripts from stalled ribosomes for their partitioning into stress granules requires the activities of VCP, components of the ribosome-associated quality control (RQC) complex, and the proteasome. This is an unexpected contribution of the RQC system in releasing mRNAs from translation under stress, thus identifying a new type of stress-activated RQC (saRQC) distinct from canonical RQC pathways in mRNA substrates, cellular context, and mRNA fate.
Next-generation RNA Sequencing–based Biomarker Characterization of Chromophobe Renal Cell Carcinoma and Related Oncocytic Neoplasms
Stephanie L. Skala, Xiaoming Wang, Yuping Zhang, Rahul Mannan, Lisha Wang, Sathiya P. Narayanan, Pankaj Vats, Fengyun Su, Jin Chen, Xuhong Cao, Javed Siddiqui, Pedram Argani, Marcin P. Cieslik, Thomas J. Giordano, Arul M. Chinnaiyan, Saravana M. Dhanasekaran, Rohit Mehra, 0302-2838/© 2020 European Association of Urology,  

Conclusions: We demonstrate a pipeline for the identification and validation of RCC subtype– specific biomarkers that can aid in the confirmation of cell of origin and may facilitate accurate classification and diagnosis of renal tumors. 
Comba A, Dunn PJ, Argento AE, Kadiyala P, Ventosa M, Patel P, Zamler DB, Núñez FJ, Zhao L, Castro MG, Lowenstein PR. Neuro Oncol.   2020 Jun 9;22(6):806-818.

Conclusions: Gliomas employ Fyn mediated mechanisms to enhance immune suppression and promote tumor progression. We propose that Fyn inhibition within glioma cells could improve the efficacy of antiglioma immunotherapies.
Single Cell Transcriptomics Analysis Identifies Nuclear Protein 1 as a Regulator of Docetaxel Resistance in Prostate Cancer Cells
Patricia M Schnepp, Greg Shelley, Jinlu Dai, Nicole Wakim, Hui Jiang, Atsushi Mizokami and 
Evan T. Keller, Mol Cancer Res  June 8 2020,  doi: 10.1158/1541-7786.MCR-20-0051

Collectively, these data demonstrate the utility of scRNA-seq to identify regulators of drug resistance. Furthermore, NUPR1 was identified as a mediator of PCa drug resistance, which provides the rationale to explore NUPR1 and its target genes to for reversal of docetaxel resistance. Implications: Using single cell sequencing of PCa, we show that NUPR1 plays a role in docetaxel resistance.