The RNA Transcript, September 21, 2020
TODAY, Monday,
Sept. 21, 4:00–5:00

Our seminar series features:

Pennsylvania State University

“Structures of eukaryotic RNases MRP/P RNPs reveal RNA-driven protein remodeling”
Keywords: Ribozymes, RNase P, RNase MRP, ribonucleoprotein complexes, RNA-driven protein remodelling

Our fall seminar series goes international
This fall, our seminar series is featuring international scientific leaders.They will give virtual talks and meet with our community.
Our seminar series was launched on April 14, 2016, and featured RNA scientists within U-M. It was a great opportunity to learn about each other’s research within U-M. We have progressively expanded our horizons, and invited high-profile researchers from within the U.S. This year, we’re pleased to also have speakers from around the world and, again, a remarkable line-up!
On the occasion of the National Postdoc Appreciation Week, the University of Michigan (U-M) Center for RNA Biomedicine’s community warmly thanks UM’s postdocs for their tremendous contributions to the field of RNA research and to the life of our scientific community.

According to the U-M Postdoctoral Association (UMPDA), an estimated 1,400 to 1,600 postdocs work across the University. They are key contributors to U-M’s research endeavor and to the dynamics of our labs. To thank them all, we interviewed four of them, all passionate about their research and lab work, representative of the entire cohort. Here is a summary of what they had to say about their roles at the U-M.

READ what they had to say about their roles at U-M.
Monday, September 21, 4:00 pm, | U-M Center for RNA Biomedicine, Seminar Series

Andrey Krasilnikov, Pennsylvania State University
“Structures of eukaryotic RNases MRP/P RNPs reveal RNA-driven protein remodeling”
Wednesday–Friday, September 23–25, 2020, | RIBOCLUB Symposium

"The Current Pandemic and Beyond" 
Wednesday, September 23, 8:45 am–3:15 pm | U-M 2020 Precision Health Symposium

This year’s event focuses on the important role of community participants in research and the positive impact research can have on communities. Featuring national and local experts from a variety of precision health disciplines, this event will provide thought-provoking sessions from multiple perspectives.

Keynote Speaker: Nancy Cox, Ph.D., Vanderbilt University
Thursday–Friday, September 24–25, 2020 | National Cancer Institute (NCI)
Note that registration closes on September 22, 2020 at 5:00 PM EDT

NCI workshop on the topic of RNA modifications and cancer
"The Role of Epitranscriptomic Modifications in the Biology of Cancer Workshop"

Worshop Chair: Dr. Richard Gregory, Professor in the Department of Biological Chemistry and Molecular Pharmacology, and Department of Pediatrics, Boston Children’s Hospital, Harvard Medical School, and Harvard Stem Cell Institute
Thursday, September 24, 4:00–5:00 pm | Center's Journal Club

Reviewed publication:
“Ribosomal Formation of Thioamide Bonds in Polypeptide Synthesis” Rumit Maini, Hiroyuki Kimura, Ryo Takatsuji, Takayuki Katoh, Yuki Goto, and Hiroaki Suga 

Monday, September 28, 9:00–10:00 am | U-M Center for RNA Biomedicine, Seminar Series

"Genetic code reprogramming that Revolutionizes the discovery processes of peptide drug leads"

Keywords: Ribozymes, Flexizymes, Peptides, Genetic code, Translation, Drugs
Monday, October 5, 4:00–5:00 pm | U-M Center for RNA Biomedicine, Seminar Series

Chase Weidmann, Ph.D., Washington University School of Medicine in St. Louis

Our members' publications are available through Altmetric. Five queries are currently available: "RNA," "microRNA," "Transcriptome," "Translation," and "Molecule." Please make sure to have at least one of these key words in your title or abstract. Below are recent highlights.
Principles of mRNA control by human PUM proteins elucidated from multi-modal experiments and integrative data analysis Michael B WolfeTrista L SchagatMichelle
T Paulsen, Brian MagnusonMats Ljungman, Daeyoon ParkChi ZhangZachary T CampbellAaron C Goldstrohm and Peter L Freddolino, RNA, a publication of The RNA Society, August 4, 2020, doi:10.1261/rna.077362.120

By integrating our results with prior knowledge, we developed a ‘rulebook’ of key contextual features that differentiate functional vs. non-functional PREs, allowing us to train machine learning models that accurately predict the functional regulation of RNA targets by the human PUM proteins.
Single-Cell Transcriptome Analysis of Colon Cancer Cell Response to 5-Fluorouracil-Induced DNA Damage, Sung Rye Park, Sim Namkoong, Leon Friesen, Chun-Seok Cho, Zac Zezhi Zhang, Yu-Chih Chen, Euisik Yoon, Chang H.Kim, Hojoong Kwak, Hyun Min Kang, Jun Hee Lee, Cell Reports, Volume 32, Issue 8, 25 August 2020, 108077,

  • Single-cell transcriptome responses to 5FU-induced DNA damage are characterized
  • Transcriptome phenotypes underlying heterogeneous 5FU responses are identified
  • Cell-fate-specific gene expression patterns after 5FU treatment are shown
  • Key observations are reproduced in flow cytometry and other DNA damage treatments
Magnetic resonance imaging of human neural stem cells in rodent and primate brain, Lisa M. McGinley, Matthew S. Willsey, Osama N. Kashlan, Kevin S. Chen, John M. Hayes, Ingrid L. Bergin, Shayna N. Mason, Aaron W. Stebbins, Jacquelin F. Kwentus, Crystal Pacut, Jennifer Kollmer, Stacey A. Sakowski, Caleb B. Bell, Cynthia A. Chestek, Geoffrey G. Murphy, Parag G. Patil, Eva L. Feldman, Stem Cells Translational Medicine, August 2020

Overall, the findings of this study indicate that magneto‐endosymbionts are an effective tool for in vivo monitoring of cell‐based therapies with potential relevance to many neurological applications.
Direct kinetic fingerprinting and digital counting of single protein molecules, Tanmay Chatterjee, Achim Knappik, Erin Sandford, Muneesh Tewari, Sung Won Choi, William B. Strong, Evan P. Thrush, Kenneth J. Oh, Ning Liu, Nils G. Walter, and  Alexander Johnson-Buck, PNAS, August 31, 2020

Illustration: Kinetic fingerprinting with dynamically binding fluorescent antibodies (cyan with red glowing orb) distinguishes between the repetitive binding to a specific target protein (left) and the less repetitive nonspecific binding to other proteins (right) captured on a surface using conventional IgG antibodies (blue Y-shaped molecules, bottom). The highly specific single-molecule detection of this approach provides approximately 100-fold higher sensitivity than conventional protein detection assays such as ELISA.