Water Detect Coliforms is a chromogenic medium used for the simultaneous detection of total coliforms and Escherichia coli in the microbiological monitoring of water quality.

Tryptose provides nitrogen, carbon, vitamins and minerals. Sodium Chloride maintains the osmotic balance of the medium. Sorbitol and IPTG are the fermentable carbohydrates. Tryptophan is added to improve the indole reaction. Potassium phosphates act as a buffer. Sodium lauryl sulfate inhibits organisms other than coliform bacteria. X-GAL is the chromogenic substrate cleaved by coliforms. MUG is the fluorogenic substrate highly specific for E. coli.